TY - JOUR
T1 - The verification of a mammalian toxicant classification using a mosquito screening method
AU - Richie, John P.
AU - Mills, Betty Jane
AU - Lang, Calvin A.
N1 - Funding Information:
This work was supported by a grant from the US. Environmental Protection Agency. The authors wish to thank Dr. Theresa Chen for generous advice and Mrs. Helen Shields and Ms. Yi-Yi Tsai for their technical assistance.
PY - 1984/12
Y1 - 1984/12
N2 - The Verification of a Mammalian Toxicant Classification Using a Mosquito Screening Method. RICHIE, J. P., JR., MILLS, B. J., AND LANG, C A. (1984). Fundam. Appl. Toxicol. 4, 1029-1035. Routine toxicity screening of substances using rodents can be time consuming and expensive. For these reasons various alternative methods have been developed, but many lack standardization and acceptance. It was hypothesized that a satisfactory classification of mammalian toxicants could be obtained using a simple mosquito (Aedes aegypti) screening method. To this end, median lethal concentration (LC50) values were determined for a wide variety of mammalian toxicants representing a range of toxicity classes. Each substance was assayed in aqueous solution by incubation for different times, up to 24 hr, using at least 30 individual larvae and six different concentrations, for valid statistical analysis. With few exceptions, the resultant toxicity classes were the same as those determined using mammalian models. This classification was validated further by the very high correlation (r = 0.97) determined between the mosquito LC50 values and the rat LD50 values. These results suggest that this mosquito method can be used to rapidly screen compounds of potential mammalian toxicity.
AB - The Verification of a Mammalian Toxicant Classification Using a Mosquito Screening Method. RICHIE, J. P., JR., MILLS, B. J., AND LANG, C A. (1984). Fundam. Appl. Toxicol. 4, 1029-1035. Routine toxicity screening of substances using rodents can be time consuming and expensive. For these reasons various alternative methods have been developed, but many lack standardization and acceptance. It was hypothesized that a satisfactory classification of mammalian toxicants could be obtained using a simple mosquito (Aedes aegypti) screening method. To this end, median lethal concentration (LC50) values were determined for a wide variety of mammalian toxicants representing a range of toxicity classes. Each substance was assayed in aqueous solution by incubation for different times, up to 24 hr, using at least 30 individual larvae and six different concentrations, for valid statistical analysis. With few exceptions, the resultant toxicity classes were the same as those determined using mammalian models. This classification was validated further by the very high correlation (r = 0.97) determined between the mosquito LC50 values and the rat LD50 values. These results suggest that this mosquito method can be used to rapidly screen compounds of potential mammalian toxicity.
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U2 - 10.1093/toxsci/4.6.1029
DO - 10.1093/toxsci/4.6.1029
M3 - Article
C2 - 6519371
AN - SCOPUS:77957180490
VL - 4
SP - 1029
EP - 1035
JO - Toxicological Sciences
JF - Toxicological Sciences
SN - 1096-6080
IS - 6
ER -