The yan gene is highly conserved in Drosophila and its expression suggests a complex role throughout development

Mitch D. Price, Zhi-chun Lai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Competence for cell fate determination and cellular differentiation is under tight control of regulatory genes. Yan, a nuclear target of receptor tyrosine kinase (RTK signaling, is an E twenty six (ETS) DNA-binding protein that functions as a negative regulator of cell differentiation and proliferation in Drosophila. Most members of RTK signaling pathways are highly conserved through evolution, yet no yan orthologues have been identified to date in vertebrates. To investigate the degree of yan conservation during evolution, we have characterized a yan homologue from a sibling species of D. melanogaster, D. virilis. Our results show that the organization, primary structure and expression pattern of yan are highly conserved. Both genes span over 20 kb and contain four exons with introns at identical positions. The areas with highest amino acid similarity include the Pointed and ETS domain but there are other discrete regions with a high degree of similarity. Phylogenetic analysis reveals that yan's closest relative is the human tel gene, a negative regulator of differentiation in hematopoetic precursors. In both species, Yan is dynamically expressed beginning as early as stage 4/5 and persisting throughout embryogenesis. In third instar larvae, Yan is expressed in and behind the morphogenetic furrow of the eye imaginal disc as well as in the laminar precursor cells of the brain. Ovarian follicle cells also contain Yan protein. Conservation of the structure and expression patterns of yan genes strongly suggests that regulatory mechanisms for their expression are also conserved in these two species.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalDevelopment Genes and Evolution
Volume209
Issue number4
DOIs
StatePublished - Apr 6 1999

Fingerprint

Drosophila
gene
Genes
Imaginal Discs
imaginal discs
Melanogaster
DNA-binding proteins
Ovarian Follicle
genes
sibling species
ovarian follicles
DNA-Binding Proteins
cells
Regulator Genes
Cytoplasmic and Nuclear Receptors
regulator genes
furrows
cell differentiation
Protein-Tyrosine Kinases
Mental Competency

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

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title = "The yan gene is highly conserved in Drosophila and its expression suggests a complex role throughout development",
abstract = "Competence for cell fate determination and cellular differentiation is under tight control of regulatory genes. Yan, a nuclear target of receptor tyrosine kinase (RTK signaling, is an E twenty six (ETS) DNA-binding protein that functions as a negative regulator of cell differentiation and proliferation in Drosophila. Most members of RTK signaling pathways are highly conserved through evolution, yet no yan orthologues have been identified to date in vertebrates. To investigate the degree of yan conservation during evolution, we have characterized a yan homologue from a sibling species of D. melanogaster, D. virilis. Our results show that the organization, primary structure and expression pattern of yan are highly conserved. Both genes span over 20 kb and contain four exons with introns at identical positions. The areas with highest amino acid similarity include the Pointed and ETS domain but there are other discrete regions with a high degree of similarity. Phylogenetic analysis reveals that yan's closest relative is the human tel gene, a negative regulator of differentiation in hematopoetic precursors. In both species, Yan is dynamically expressed beginning as early as stage 4/5 and persisting throughout embryogenesis. In third instar larvae, Yan is expressed in and behind the morphogenetic furrow of the eye imaginal disc as well as in the laminar precursor cells of the brain. Ovarian follicle cells also contain Yan protein. Conservation of the structure and expression patterns of yan genes strongly suggests that regulatory mechanisms for their expression are also conserved in these two species.",
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The yan gene is highly conserved in Drosophila and its expression suggests a complex role throughout development. / Price, Mitch D.; Lai, Zhi-chun.

In: Development Genes and Evolution, Vol. 209, No. 4, 06.04.1999, p. 207-217.

Research output: Contribution to journalArticle

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