The yeast genome undergoes significant topological reorganization in quiescence

Mark T. Rutledge, Mariano Russo, Jon Matthew Belton, Job Dekker, James R. Broach

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have examined the three-dimensional organization of the yeast genome during quiescence by a chromosome capture technique as ameans of understanding how genome organization changes during development. For exponentially growing cells we observe high levels of inter-centromeric interaction but otherwise a predominance of intrachromosomal interactions over interchromosomal interactions, consistent with aggregation of centromeres at the spindle pole body and compartmentalization of individual chromosomes within the nucleoplasm. Three major changes occur in the organization of the quiescent cell genome. First, intrachromosomal associations increase at longer distances in quiescence as compared to growing cells. This suggests that chromosomes undergo condensation in quiescence, which we confirmed by microscopy by measurement of the intrachromosomal distances between two sites on one chromosome. This compaction in quiescence requires the condensin complex. Second, inter-centromeric interactions decrease, consistent with prior data indicating that centromeres disperse along an array of microtubules during quiescence. Third, inter-telomeric interactions significantly increase in quiescence, an observation also confirmed by direct measurement. Thus, survival during quiescence is associated with substantial topological reorganization of the genome.

Original languageEnglish (US)
Pages (from-to)8299-8313
Number of pages15
JournalNucleic Acids Research
Volume43
Issue number17
DOIs
StatePublished - Sep 30 2015

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Chromosomes
Yeasts
Genome
Centromere
Spindle Pole Bodies
Microtubules
Microscopy
Observation

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Rutledge, Mark T. ; Russo, Mariano ; Belton, Jon Matthew ; Dekker, Job ; Broach, James R. / The yeast genome undergoes significant topological reorganization in quiescence. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 17. pp. 8299-8313.
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The yeast genome undergoes significant topological reorganization in quiescence. / Rutledge, Mark T.; Russo, Mariano; Belton, Jon Matthew; Dekker, Job; Broach, James R.

In: Nucleic Acids Research, Vol. 43, No. 17, 30.09.2015, p. 8299-8313.

Research output: Contribution to journalArticle

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