The yeast plasmid 2μ circle encodes components required for its high copy propagation

M. Jayaram, Y. Y. Li, J. R. Broach

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

The yeast plasmid 2μ and certain hybrid plasmids constructed from it are maintained stably and at high copy number in yeast cells. By examining various mutant hybrid 2μ plasmids, we show that these properties require the integrity of four plasmid loci. Two of these, designated REPI and REP2, are active in trans and correspond to two open coding regions of 2μ. The other two loci are active only in cis and correspond to the origin of replication and to a region, designated REP3, located several hundred bp away from the origin and consisting of direct repeats of a 62 bp sequence. We propose that the REP loci constitute a copy control system that overrides normal cellular restriction on plasmid replication and amplifies the plasmid when copy number is low.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalCell
Volume34
Issue number1
DOIs
StatePublished - Aug 1983

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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