Thermal Unfolding Pathway of PHD2 Catalytic Domain in Three Different PHD2 Species: Computational Approaches

Hamid Hadi-Alijanvand, Elizabeth Anne Proctor, Bahram Goliaei, Nikolay Dokholyan, Ali A. Moosavi-Movahedi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Prolyl hydroxylase domain 2 containing protein (PHD2) is a key protein in regulation of angiogenesis and metastasis. In normoxic condition, PHD2 triggers the degradation of hypoxia-inducible factor 1 (HIF-1α) that induces the expression of hypoxia response genes. Therefore the correct function of PHD2 would inhibit angiogenesis and consequent metastasis of tumor cells in normoxic condition. PHD2 mutations were reported in some common cancers. However, high levels of HIF-1α protein were observed even in normoxic metastatic tumors with normal expression of wild type PHD2. PHD2 malfunctions due to protein misfolding may be the underlying reason of metastasis and invasion in such cases. In this study, we scrutinize the unfolding pathways of the PHD2 catalytic domain's possible species and demonstrate the properties of their unfolding states by computational approaches. Our study introduces the possibility of aggregation disaster for the prominent species of PHD2 during its partial unfolding. This may justify PHD2 inability to regulate HIF-1α level in some normoxic tumor types.

Original languageEnglish (US)
Article numbere47061
JournalPloS one
Volume7
Issue number10
DOIs
StatePublished - Oct 15 2012

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procollagen-proline dioxygenase
Prolyl Hydroxylases
active sites
Catalytic Domain
Hot Temperature
heat
Proteins
proteins
Hypoxia-Inducible Factor 1
metastasis
normoxia
Tumors
Neoplasm Metastasis
angiogenesis
Neoplasms
neoplasms
Protein Domains
Disasters
disasters
protein degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Prolyl hydroxylase domain 2 containing protein (PHD2) is a key protein in regulation of angiogenesis and metastasis. In normoxic condition, PHD2 triggers the degradation of hypoxia-inducible factor 1 (HIF-1α) that induces the expression of hypoxia response genes. Therefore the correct function of PHD2 would inhibit angiogenesis and consequent metastasis of tumor cells in normoxic condition. PHD2 mutations were reported in some common cancers. However, high levels of HIF-1α protein were observed even in normoxic metastatic tumors with normal expression of wild type PHD2. PHD2 malfunctions due to protein misfolding may be the underlying reason of metastasis and invasion in such cases. In this study, we scrutinize the unfolding pathways of the PHD2 catalytic domain's possible species and demonstrate the properties of their unfolding states by computational approaches. Our study introduces the possibility of aggregation disaster for the prominent species of PHD2 during its partial unfolding. This may justify PHD2 inability to regulate HIF-1α level in some normoxic tumor types.",
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Thermal Unfolding Pathway of PHD2 Catalytic Domain in Three Different PHD2 Species : Computational Approaches. / Hadi-Alijanvand, Hamid; Proctor, Elizabeth Anne; Goliaei, Bahram; Dokholyan, Nikolay; Moosavi-Movahedi, Ali A.

In: PloS one, Vol. 7, No. 10, e47061, 15.10.2012.

Research output: Contribution to journalArticle

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