Thermally-controlled extrusion-based bioprinting of collagen

Kazim K. Moncal, Veli Ozbolat, Pallab Datta, Dong N. Heo, Ibrahim Tarik Ozbolat

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Thermally-crosslinked hydrogels in bioprinting have gained increasing attention due to their ability to undergo tunable crosslinking by modulating the temperature and time of crosslinking. In this paper, we present a new bioink composed of collagen type-I and Pluronic® F-127 hydrogels, which was bioprinted using a thermally-controlled bioprinting unit. Bioprintability and rheology of the composite bioink was studied in a thorough manner in order to determine the optimal bioprinting time and extrusion profile of the bioink for fabrication of three-dimensional (3D) constructs, respectively. It was observed that collagen fibers aligned themselves along the directions of the printed filaments after bioprinting based on the results on an anisotropy study. Furthermore, rat bone marrow-derived stem cells (rBMSCs) were bioprinted in order to determine the effect of thermally-controlled extrusion process. In vitro viability and proliferation study revealed that rBMSCs were able to maintain their viability after extrusion and attached to collagen fibers, spread and proliferated within the constructs up to seven days of culture. [Figure not available: see fulltext.].

Original languageEnglish (US)
Article number55
JournalJournal of Materials Science: Materials in Medicine
Volume30
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Bioprinting
Collagen
Extrusion
Hydrogels
Stem cells
Crosslinking
Rats
Bone
Poloxamer
Stem Cells
Fibers
Bone Marrow
Collagen Type I
Rheology
Anisotropy
Fabrication
Composite materials
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Moncal, Kazim K. ; Ozbolat, Veli ; Datta, Pallab ; Heo, Dong N. ; Ozbolat, Ibrahim Tarik. / Thermally-controlled extrusion-based bioprinting of collagen. In: Journal of Materials Science: Materials in Medicine. 2019 ; Vol. 30, No. 5.
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Thermally-controlled extrusion-based bioprinting of collagen. / Moncal, Kazim K.; Ozbolat, Veli; Datta, Pallab; Heo, Dong N.; Ozbolat, Ibrahim Tarik.

In: Journal of Materials Science: Materials in Medicine, Vol. 30, No. 5, 55, 01.05.2019.

Research output: Contribution to journalArticle

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