Thermospray high‐performance liquid chromatography/mass spectrometric determination of cyclosporins

J. Abián; A. Stone; M. G. Morrow; M. H. Creer; L. M. Fink; J. O. Lay

doi:10.1002/rcm.1290061111

Thermospray high‐performance liquid chromatography/mass spectrometric determination of cyclosporins

J. Abián, A. Stone, M. G. Morrow, M. H. Creer, L. M. Fink, J. O. Lay

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The cylic undecapeptides cyclosporin (Csp) A, CspD and dihydro CspC (HCspC) were analyzed by high‐performance liquid chromatography/thermospray‐mass spectrometry (HPLC/TSP‐MS) on line with 1–2 pmol injected on column. Mass spectra were characterized by signals corresponding to the [M + H]⁺ ion as well as fragment ions derived from he loss of 112 (CspA and CspD) or 44, 114 and 114+44 (HCspC) mass units from the parent ion. The same qualitative profile was observed for negative‐ion acquisition where the ions were formed by proton abstraction. The application of the technique to the characterization of CspA and its major hydroxylated and dealkylated metabolites in human blood samples is presented.

Original language	English (US)
Pages (from-to)	684-689
Number of pages	6
Journal	Rapid Communications in Mass Spectrometry
Volume	6
Issue number	11
DOIs	https://doi.org/10.1002/rcm.1290061111
State	Published - Nov 1992

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Spectroscopy
Organic Chemistry

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title = "Thermospray high‐performance liquid chromatography/mass spectrometric determination of cyclosporins",

abstract = "The cylic undecapeptides cyclosporin (Csp) A, CspD and dihydro CspC (HCspC) were analyzed by high‐performance liquid chromatography/thermospray‐mass spectrometry (HPLC/TSP‐MS) on line with 1–2 pmol injected on column. Mass spectra were characterized by signals corresponding to the [M + H]+ ion as well as fragment ions derived from he loss of 112 (CspA and CspD) or 44, 114 and 114+44 (HCspC) mass units from the parent ion. The same qualitative profile was observed for negative‐ion acquisition where the ions were formed by proton abstraction. The application of the technique to the characterization of CspA and its major hydroxylated and dealkylated metabolites in human blood samples is presented.",

author = "J. Abi{\'a}n and A. Stone and Morrow, {M. G.} and Creer, {M. H.} and Fink, {L. M.} and Lay, {J. O.}",

year = "1992",

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T1 - Thermospray high‐performance liquid chromatography/mass spectrometric determination of cyclosporins

AU - Abián, J.

AU - Stone, A.

AU - Morrow, M. G.

AU - Creer, M. H.

AU - Fink, L. M.

AU - Lay, J. O.

PY - 1992/11

Y1 - 1992/11

N2 - The cylic undecapeptides cyclosporin (Csp) A, CspD and dihydro CspC (HCspC) were analyzed by high‐performance liquid chromatography/thermospray‐mass spectrometry (HPLC/TSP‐MS) on line with 1–2 pmol injected on column. Mass spectra were characterized by signals corresponding to the [M + H]+ ion as well as fragment ions derived from he loss of 112 (CspA and CspD) or 44, 114 and 114+44 (HCspC) mass units from the parent ion. The same qualitative profile was observed for negative‐ion acquisition where the ions were formed by proton abstraction. The application of the technique to the characterization of CspA and its major hydroxylated and dealkylated metabolites in human blood samples is presented.

AB - The cylic undecapeptides cyclosporin (Csp) A, CspD and dihydro CspC (HCspC) were analyzed by high‐performance liquid chromatography/thermospray‐mass spectrometry (HPLC/TSP‐MS) on line with 1–2 pmol injected on column. Mass spectra were characterized by signals corresponding to the [M + H]+ ion as well as fragment ions derived from he loss of 112 (CspA and CspD) or 44, 114 and 114+44 (HCspC) mass units from the parent ion. The same qualitative profile was observed for negative‐ion acquisition where the ions were formed by proton abstraction. The application of the technique to the characterization of CspA and its major hydroxylated and dealkylated metabolites in human blood samples is presented.

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Thermospray high‐performance liquid chromatography/mass spectrometric determination of cyclosporins

Abstract

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