Three brain sodium channel α-subunit genes are clustered on the proximal segment of mouse chromosome 2

Danielle Malo, Erwin Schurr, Julia Dorfman, Victor Canfield, Robert Levenson, Philippe Gros

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

We have used long-range physical mapping and restriction fragment length polymorphisms between two mouse species to determine the chromosomal organization and location of the genes encoding three distinct isoforms of the α-subunit of the brain sodium channel. Physical mapping by pulsed-field gel electrophoresis has established that Scn2a and Scn3a (genes encoding type II and type III sodium channel α-subunit isoforms) are physically linked and are separated by a maximum distance of 600 kb. The segregation of restriction fragment length variations in backcross progeny of a Mus musculus and Mus spretus mating indicates that Scn1a (gene encoding the type I sodium channel α subunit) and Scn2a are tightly linked and are separated by a distance of 0.7 cM. Linkage analysis in backcross and recombinant inbred (BXD and AKXD) strains of mice localized the three sodium channel genes to the proximal segment of mouse chromosome 2 and suggested the probable gene order centromere-Hc-Neb-Pmv7-Scn2a/Scn3a-Scn1a-Mpmv14. These results indicate that the three isoforms of the brain sodium channel α-subunit are encoded by three distinct genes that share a common ancestral origin.

Original languageEnglish (US)
Pages (from-to)666-672
Number of pages7
JournalGenomics
Volume10
Issue number3
DOIs
StatePublished - Jul 1991

All Science Journal Classification (ASJC) codes

  • Genetics

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