Three novel herpesviruses associated with stomatitis in Sudan plated lizards (Gerrhosaurus major) and a black-lined plated lizard (Gerrhosaurus nigrolineatus)

James F.X. Wellehan, Donald K. Nichols, Lingling Li, Vivek Kapur

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Glossal stomatitis was observed in a Sudan plated lizard (Gerrhosaurus major) with severe dyspnea. On necropsy, intranuclear inclusion bodies were seen in the periglottal lingual epithelium. Labial stomatitis was seen in a second Sudan plated lizard and a black-lined plated lizard (G. nigrolineatus). Degenerate polymerase chain reaction (PCR) primers targeting a conserved region of herpesvirus DNA-dependent DNA polymerase gene were used to amplify products from lesions from each lizard. Nucleotide sequencing of the PCR products showed that the sequence from each lizard was unique. Phylogenetic and comparative sequence analyses suggest that these viruses are novel members of the subfamily Alphaherpesvirinae, and they are here termed gerrhosaurid herpesviruses 1-3. Results of our analyses suggest that the genus Gerrhosaurus can be infected by these novel herpesviruses.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalJournal of Zoo and Wildlife Medicine
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

Stomatitis
Sudan
Lizards
Herpesviridae
lizards
tongue
Tongue
Alphaherpesvirinae
polymerase chain reaction
nuclear inclusions
Intranuclear Inclusion Bodies
Polymerase Chain Reaction
dyspnea
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
lips
Lip
lesions (animal)
Dyspnea
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Cite this

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abstract = "Glossal stomatitis was observed in a Sudan plated lizard (Gerrhosaurus major) with severe dyspnea. On necropsy, intranuclear inclusion bodies were seen in the periglottal lingual epithelium. Labial stomatitis was seen in a second Sudan plated lizard and a black-lined plated lizard (G. nigrolineatus). Degenerate polymerase chain reaction (PCR) primers targeting a conserved region of herpesvirus DNA-dependent DNA polymerase gene were used to amplify products from lesions from each lizard. Nucleotide sequencing of the PCR products showed that the sequence from each lizard was unique. Phylogenetic and comparative sequence analyses suggest that these viruses are novel members of the subfamily Alphaherpesvirinae, and they are here termed gerrhosaurid herpesviruses 1-3. Results of our analyses suggest that the genus Gerrhosaurus can be infected by these novel herpesviruses.",
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