Thy-1 antigen in murine hematopoietic progenitor differentiation.

Barbara Miller, J. M. Lipton

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Until recently, the presence of Thy-1 antigen was thought to identify thymus-derived lymphocytes as opposed to other lymphohematopoietic cells. With the use of a highly amplified immunofluorescence assay and a monoclonal anti-Thy-1 antibody, the Thy-1 antigen has been detected on CFU-S. To determine the role of Thy-1 antigen in the differentiation of more committed hematopoietic progenitors, murine bone marrow was treated with anti-Thy-1.2 antibody and complement and cultured. Growth of erythroid burst-forming units (BFU-E) and granulocyte/macrophage colony-forming units (CFU-G/M) was greatly reduced following antibody and complement treatment and could not be restored with mitogen-stimulated spleen cell supernatants, splenic T cells, or thymocytes. These data demonstrate that a Thy-1.2-bearing cell is important in differentiation of early murine hematopoietic progenitors and they provide evidence that anti-Thy-1.2 antibody and complement treatment removes Thy-1.2 antigen-bearing hematopoietic progenitors. In contrast, growth of the macrophage progenitor (CFU-M) stimulated by L cell-conditioned media and the more mature erythroid colony-forming unit (CFU-E) was unaffected by anti-Thy-1.2 antibody and complement treatment. Therefore, Thy-1.2 antigen is not present on these progenitors. These data suggest that Thy-1.2 antigen is a differentiation antigen that is present on early murine hematopoietic progenitors and is lost as they differentiate.

Original languageEnglish (US)
Pages (from-to)389-397
Number of pages9
JournalProgress in Clinical and Biological Research
Volume134
StatePublished - Dec 1 1983

Fingerprint

Thy-1 Antigens
Erythroid Precursor Cells
Granulocyte-Macrophage Progenitor Cells
Differentiation Antigens
Thymocytes
Conditioned Culture Medium
Growth
Mitogens
Thymus Gland
Fluorescent Antibody Technique
Therapeutics
Spleen
Bone Marrow
Macrophages
anti-Thy antibody
Lymphocytes
T-Lymphocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{f9b22c738baa4ac0bc31c192cbd4b062,
title = "Thy-1 antigen in murine hematopoietic progenitor differentiation.",
abstract = "Until recently, the presence of Thy-1 antigen was thought to identify thymus-derived lymphocytes as opposed to other lymphohematopoietic cells. With the use of a highly amplified immunofluorescence assay and a monoclonal anti-Thy-1 antibody, the Thy-1 antigen has been detected on CFU-S. To determine the role of Thy-1 antigen in the differentiation of more committed hematopoietic progenitors, murine bone marrow was treated with anti-Thy-1.2 antibody and complement and cultured. Growth of erythroid burst-forming units (BFU-E) and granulocyte/macrophage colony-forming units (CFU-G/M) was greatly reduced following antibody and complement treatment and could not be restored with mitogen-stimulated spleen cell supernatants, splenic T cells, or thymocytes. These data demonstrate that a Thy-1.2-bearing cell is important in differentiation of early murine hematopoietic progenitors and they provide evidence that anti-Thy-1.2 antibody and complement treatment removes Thy-1.2 antigen-bearing hematopoietic progenitors. In contrast, growth of the macrophage progenitor (CFU-M) stimulated by L cell-conditioned media and the more mature erythroid colony-forming unit (CFU-E) was unaffected by anti-Thy-1.2 antibody and complement treatment. Therefore, Thy-1.2 antigen is not present on these progenitors. These data suggest that Thy-1.2 antigen is a differentiation antigen that is present on early murine hematopoietic progenitors and is lost as they differentiate.",
author = "Barbara Miller and Lipton, {J. M.}",
year = "1983",
month = "12",
day = "1",
language = "English (US)",
volume = "134",
pages = "389--397",
journal = "Progress in Clinical and Biological Research",
issn = "0361-7742",
publisher = "John Wiley and Sons Inc.",

}

Thy-1 antigen in murine hematopoietic progenitor differentiation. / Miller, Barbara; Lipton, J. M.

In: Progress in Clinical and Biological Research, Vol. 134, 01.12.1983, p. 389-397.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thy-1 antigen in murine hematopoietic progenitor differentiation.

AU - Miller, Barbara

AU - Lipton, J. M.

PY - 1983/12/1

Y1 - 1983/12/1

N2 - Until recently, the presence of Thy-1 antigen was thought to identify thymus-derived lymphocytes as opposed to other lymphohematopoietic cells. With the use of a highly amplified immunofluorescence assay and a monoclonal anti-Thy-1 antibody, the Thy-1 antigen has been detected on CFU-S. To determine the role of Thy-1 antigen in the differentiation of more committed hematopoietic progenitors, murine bone marrow was treated with anti-Thy-1.2 antibody and complement and cultured. Growth of erythroid burst-forming units (BFU-E) and granulocyte/macrophage colony-forming units (CFU-G/M) was greatly reduced following antibody and complement treatment and could not be restored with mitogen-stimulated spleen cell supernatants, splenic T cells, or thymocytes. These data demonstrate that a Thy-1.2-bearing cell is important in differentiation of early murine hematopoietic progenitors and they provide evidence that anti-Thy-1.2 antibody and complement treatment removes Thy-1.2 antigen-bearing hematopoietic progenitors. In contrast, growth of the macrophage progenitor (CFU-M) stimulated by L cell-conditioned media and the more mature erythroid colony-forming unit (CFU-E) was unaffected by anti-Thy-1.2 antibody and complement treatment. Therefore, Thy-1.2 antigen is not present on these progenitors. These data suggest that Thy-1.2 antigen is a differentiation antigen that is present on early murine hematopoietic progenitors and is lost as they differentiate.

AB - Until recently, the presence of Thy-1 antigen was thought to identify thymus-derived lymphocytes as opposed to other lymphohematopoietic cells. With the use of a highly amplified immunofluorescence assay and a monoclonal anti-Thy-1 antibody, the Thy-1 antigen has been detected on CFU-S. To determine the role of Thy-1 antigen in the differentiation of more committed hematopoietic progenitors, murine bone marrow was treated with anti-Thy-1.2 antibody and complement and cultured. Growth of erythroid burst-forming units (BFU-E) and granulocyte/macrophage colony-forming units (CFU-G/M) was greatly reduced following antibody and complement treatment and could not be restored with mitogen-stimulated spleen cell supernatants, splenic T cells, or thymocytes. These data demonstrate that a Thy-1.2-bearing cell is important in differentiation of early murine hematopoietic progenitors and they provide evidence that anti-Thy-1.2 antibody and complement treatment removes Thy-1.2 antigen-bearing hematopoietic progenitors. In contrast, growth of the macrophage progenitor (CFU-M) stimulated by L cell-conditioned media and the more mature erythroid colony-forming unit (CFU-E) was unaffected by anti-Thy-1.2 antibody and complement treatment. Therefore, Thy-1.2 antigen is not present on these progenitors. These data suggest that Thy-1.2 antigen is a differentiation antigen that is present on early murine hematopoietic progenitors and is lost as they differentiate.

UR - http://www.scopus.com/inward/record.url?scp=0020999829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020999829&partnerID=8YFLogxK

M3 - Article

C2 - 6141570

AN - SCOPUS:0020999829

VL - 134

SP - 389

EP - 397

JO - Progress in Clinical and Biological Research

JF - Progress in Clinical and Biological Research

SN - 0361-7742

ER -