Tick-borne encephalitis virus in northern Italy: Molecular analysis, relationships with density and seasonal dynamics of Ixodes ricinus

P. J. Hudson, A. Rizzoli, R. Rosà, C. Chemini, L. D. Jones, E. A. Gould

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Ixodes ricinus ticks were collected from dragging vegetation and from shot roe deer in the province of Trento and Belluno in northern Italy. Ticks were pooled for analyses and from 1060 pools of ticks collected in the province of Belluno and 12 390 tick samples collected in Trentino, four proved positive by immunofluorescence microscopy using a tick-borne encephalitis (TBE)-specific antiserum. The identity of the virus isolates was determined by RT-PCR cycle sequencing and they were all found to be closely similar (> 98% nucleotide identity) to typical western European TBE complex viruses as found in Austria. The isolates from Trentino differed from the Neudorfl strain of western European TBE virus at eight nucleotide positions but as these nucleotide substitutions were all synonymous, there were no amino acid changes. These results imply that the virus isolates in Trentino have changed slightly from the typical European strains isolated in nearby Austria. The abundance of questing ticks and ticks feeding on roe deer was greater in TBE positive hunting districts than in hunting districts where TBE complex viruses were only probable or believed to be absent. In TBE positive and probable districts synchrony in the seasonal dynamics of larvae and nymphs of I. ricinus was observed. This study provides evidence to suggest that roe deer may have an important role to play in the maintenance of tick density and in the persistence of TBE virus.

Original languageEnglish (US)
Pages (from-to)304-313
Number of pages10
JournalMedical and Veterinary Entomology
Volume15
Issue number3
DOIs
StatePublished - Oct 9 2001

Fingerprint

Tick-borne encephalitis virus
encephalitis
Tick-Borne Encephalitis Viruses
Ixodes
Ixodes ricinus
molecular analysis
tick
Ticks
tick-borne encephalitis
Tick-Borne Encephalitis
Italy
ticks
virus
Deer
Capreolus capreolus
viruses
Nucleotides
Austria
nucleotides
deer

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

Cite this

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title = "Tick-borne encephalitis virus in northern Italy: Molecular analysis, relationships with density and seasonal dynamics of Ixodes ricinus",
abstract = "Ixodes ricinus ticks were collected from dragging vegetation and from shot roe deer in the province of Trento and Belluno in northern Italy. Ticks were pooled for analyses and from 1060 pools of ticks collected in the province of Belluno and 12 390 tick samples collected in Trentino, four proved positive by immunofluorescence microscopy using a tick-borne encephalitis (TBE)-specific antiserum. The identity of the virus isolates was determined by RT-PCR cycle sequencing and they were all found to be closely similar (> 98{\%} nucleotide identity) to typical western European TBE complex viruses as found in Austria. The isolates from Trentino differed from the Neudorfl strain of western European TBE virus at eight nucleotide positions but as these nucleotide substitutions were all synonymous, there were no amino acid changes. These results imply that the virus isolates in Trentino have changed slightly from the typical European strains isolated in nearby Austria. The abundance of questing ticks and ticks feeding on roe deer was greater in TBE positive hunting districts than in hunting districts where TBE complex viruses were only probable or believed to be absent. In TBE positive and probable districts synchrony in the seasonal dynamics of larvae and nymphs of I. ricinus was observed. This study provides evidence to suggest that roe deer may have an important role to play in the maintenance of tick density and in the persistence of TBE virus.",
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Tick-borne encephalitis virus in northern Italy : Molecular analysis, relationships with density and seasonal dynamics of Ixodes ricinus. / Hudson, P. J.; Rizzoli, A.; Rosà, R.; Chemini, C.; Jones, L. D.; Gould, E. A.

In: Medical and Veterinary Entomology, Vol. 15, No. 3, 09.10.2001, p. 304-313.

Research output: Contribution to journalArticle

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AU - Hudson, P. J.

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