@inbook{c64808a2c26b49be8dac5dbe1c426c6b,
title = "Time-Lapse Imaging of Root Pathogenesis and Fungal Proliferation Without Physically Disrupting Roots",
abstract = "Microscopic observation of root disease onset and progression is typically performed by harvesting different plants at multiple time points. This approach prevents the monitoring of individual encounter sites over time, often mechanically damages roots, and exposes roots to unnatural conditions during observation. Here, we describe a method developed to avoid these problems and its application to study Fusarium oxysporum-Arabidopsis thaliana interactions. This method enabled three-dimensional, time-lapse imaging of both A. thaliana and F. oxysporum as they interact via the use of confocal and multi-photon microscopy and facilitated inquiries about the genetic mechanism underpinning Fusarium wilt.",
author = "Kim, {Hye Seon} and Park, {Sook Young} and Seogchan Kang and Czymmek, {Kirk J.}",
note = "Funding Information: Most of the materials and associated protocols described here were developed using support from USDA-NRI (2002-02367). Generation of F. oxysporum transformants expressing YC3.60 and analyses of their Ca2+ signatures as they encounter roots and proliferate in the root vascular system were supported by the US National Science Foundation (MCB-1051667). The USDA Specialty Crop Multi-State Program (AM170200XXXXG006), the USDA National Institute of Food and Agriculture and Federal Appropriations (Project PEN04655; Accession # 1016291), and the US Department of Agriculture, Agricultural Research Service, supported the preparation of this chapter. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1795-3_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--170",
booktitle = "Methods in Molecular Biology",
}