TiO2 nanoparticle exposure and illumination during zebrafish development: Mortality at parts per billion concentrations

Ofek Bar-Ilan, Connie C. Chuang, Denise J. Schwahn, Sarah Yang, Sanjay Joshi, Joel A. Pedersen, Robert J. Hamers, Richard E. Peterson, Warren Heideman

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Abstract

Photoactivation of titanium dioxide nanoparticles (TiO2NPs) can produce reactive oxygen species (ROS). Over time, this has the potential to produce cumulative cellular damage. To test this, we exposed zebrafish (Danio rerio) to two commercial TiO2NP preparations at concentrations ranging from 0.01 to 10 000 ng/mL over a 23 day period spanning embryogenesis, larval development, and juvenile metamorphosis. Fish were illuminated with a lamp that mimics solar irradiation. TiO2NP exposure produced significant mortality at 1 ng/mL. Toxicity included stunted growth, delayed metamorphosis, malformations, organ pathology, and DNA damage. TiO 2NPs were found in the gills and gut and elsewhere. The two preparations differed in nominal particle diameter (12.1 ± 3.7 and 23.3 ± 9.8 nm) but produced aggregates in the 1 μm range. Both were taken up in a dose-dependent manner. Illuminated particles produced a time- and dose-dependent increase in 8-hydroxy-2′-deoxyguanosine DNA adducts consistent with cumulative ROS damage. Zebrafish take up TiO2NPs from the aqueous environment even at low ng/mL concentrations, and these particles when illuminated in the violet-near UV range produce cumulative toxicity.

Original languageEnglish (US)
Pages (from-to)4726-4733
Number of pages8
JournalEnvironmental Science and Technology
Volume47
Issue number9
DOIs
StatePublished - May 7 2013

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Bar-Ilan, O., Chuang, C. C., Schwahn, D. J., Yang, S., Joshi, S., Pedersen, J. A., Hamers, R. J., Peterson, R. E., & Heideman, W. (2013). TiO2 nanoparticle exposure and illumination during zebrafish development: Mortality at parts per billion concentrations. Environmental Science and Technology, 47(9), 4726-4733. https://doi.org/10.1021/es304514r