Tissue engineering scaffolds based on photocured dimethacrylate polymers for in vitro optical imaging

Forrest A. Landis, Jean S. Stephens, James A. Cooper, Marcus T. Cicerone, Sheng Lin-Gibson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Model tissue engineering scaffolds based on photocurable resin mixtures with sodium chloride have been prepared for optical imaging studies of cell attachment. A photoactivated ethoxylated bisphenol A dimethacrylate was mixed with sieved sodium chloride (NaCl) crystals and photocured to form a cross-linked composite. Upon soaking in water, the NaCl dissolved to leave a porous scaffold with desirable optical properties, mechanical integrity, and controlled porosity. Scaffolds were prepared with salt crystals that had been sieved to average diameters of 390, 300, 200, and 100 μm, yielding porosities of approximately 75 vol %. Scanning electron microscopy and X-ray microcomputed tomography confirmed that the pore size distribution of the scaffolds could be controlled using this photocuring technique. Compression tests showed that for scaffolds with 84% (by mass fraction) salt, the larger pore size scaffolds were more rigid, while the smaller pore size scaffolds were softer and more readily compressible. The prepared scaffolds were seeded with osteoblasts, cultured between 3 and 18 d, and examined using confocal microscopy. Because the cross-linked polymer in the scaffolds is an amorphous glass, it was possible to optically image cells that were over 400 μm beneath the surface of the sample.

Original languageEnglish (US)
Pages (from-to)1751-1757
Number of pages7
JournalBiomacromolecules
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2006

Fingerprint

Tissue Scaffolds
Porosity
Optical Imaging
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Sodium Chloride
Scaffolds
Polymers
Salts
Imaging techniques
X-Ray Microtomography
Osteoblasts
Confocal Microscopy
Electron Scanning Microscopy
Glass
Pore size
Water
Sodium chloride
Crystals

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Landis, Forrest A. ; Stephens, Jean S. ; Cooper, James A. ; Cicerone, Marcus T. ; Lin-Gibson, Sheng. / Tissue engineering scaffolds based on photocured dimethacrylate polymers for in vitro optical imaging. In: Biomacromolecules. 2006 ; Vol. 7, No. 6. pp. 1751-1757.
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Tissue engineering scaffolds based on photocured dimethacrylate polymers for in vitro optical imaging. / Landis, Forrest A.; Stephens, Jean S.; Cooper, James A.; Cicerone, Marcus T.; Lin-Gibson, Sheng.

In: Biomacromolecules, Vol. 7, No. 6, 01.06.2006, p. 1751-1757.

Research output: Contribution to journalArticle

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