Tissue glucose utilization during epinephrine-induced hyperglycemia

K. Meszaros, C. H. Lang, D. M. Hargrove, J. J. Spitzer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The aim of this study was to investigate glucose utilization by individual tissues during epinephrine infusion. First, the applicability of the 2-deoxyglucose (2-DG) tracer technique during in vivo hyperglycemia was investigated in model systems in vitro. Epitrochlearis muscle and spleen cells were incubated with 1.25-20 mM glucose. The discrimination against 2-[14C]DG in glucose metabolic pathways, expressed by the lumped constant, remained unchanged over this wide range of glucose concentrations. It was concluded that in vivo hyperglycemia does not preclude the application of the 2-DG method. In a series of in vivo experiments, chronically catheterized conscious rats fasted for 24 h and were infused with epinephrine (0.2 μg·kg-1·min-1), which produced a two-fold increase in plasma glucose concentration. 2-[14C]DG was injected 30 min after starting the epinephrine infusion and glucose utilization rates of individual tissues were calculated based on the concentration of phosphorylated 2-DG in samples excised at 70 min. The epinephrine infusion increased glucose utilization rates by 40-160% in hindlimb muscles, skin, ileum, liver, spleen, lung, epidydimal fat, and kidney, although no change was found in the brain. Mass action of the increased plasma glucose is likely to play an important role in the enhanced rate of glucose utilization.

Original languageEnglish (US)
Pages (from-to)1770-1775
Number of pages6
JournalJournal of Applied Physiology
Volume67
Issue number5
StatePublished - Dec 1 1989

Fingerprint

Hyperglycemia
Epinephrine
Glucose
Deoxyglucose
Spleen
Hindlimb
Metabolic Networks and Pathways
Ileum
Muscle Cells
Fats
Kidney
Muscles
Lung
Skin
Liver
Brain

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Meszaros, K., Lang, C. H., Hargrove, D. M., & Spitzer, J. J. (1989). Tissue glucose utilization during epinephrine-induced hyperglycemia. Journal of Applied Physiology, 67(5), 1770-1775.
Meszaros, K. ; Lang, C. H. ; Hargrove, D. M. ; Spitzer, J. J. / Tissue glucose utilization during epinephrine-induced hyperglycemia. In: Journal of Applied Physiology. 1989 ; Vol. 67, No. 5. pp. 1770-1775.
@article{ee25c6094b0540a899648c76871a12ed,
title = "Tissue glucose utilization during epinephrine-induced hyperglycemia",
abstract = "The aim of this study was to investigate glucose utilization by individual tissues during epinephrine infusion. First, the applicability of the 2-deoxyglucose (2-DG) tracer technique during in vivo hyperglycemia was investigated in model systems in vitro. Epitrochlearis muscle and spleen cells were incubated with 1.25-20 mM glucose. The discrimination against 2-[14C]DG in glucose metabolic pathways, expressed by the lumped constant, remained unchanged over this wide range of glucose concentrations. It was concluded that in vivo hyperglycemia does not preclude the application of the 2-DG method. In a series of in vivo experiments, chronically catheterized conscious rats fasted for 24 h and were infused with epinephrine (0.2 μg·kg-1·min-1), which produced a two-fold increase in plasma glucose concentration. 2-[14C]DG was injected 30 min after starting the epinephrine infusion and glucose utilization rates of individual tissues were calculated based on the concentration of phosphorylated 2-DG in samples excised at 70 min. The epinephrine infusion increased glucose utilization rates by 40-160{\%} in hindlimb muscles, skin, ileum, liver, spleen, lung, epidydimal fat, and kidney, although no change was found in the brain. Mass action of the increased plasma glucose is likely to play an important role in the enhanced rate of glucose utilization.",
author = "K. Meszaros and Lang, {C. H.} and Hargrove, {D. M.} and Spitzer, {J. J.}",
year = "1989",
month = "12",
day = "1",
language = "English (US)",
volume = "67",
pages = "1770--1775",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "5",

}

Meszaros, K, Lang, CH, Hargrove, DM & Spitzer, JJ 1989, 'Tissue glucose utilization during epinephrine-induced hyperglycemia', Journal of Applied Physiology, vol. 67, no. 5, pp. 1770-1775.

Tissue glucose utilization during epinephrine-induced hyperglycemia. / Meszaros, K.; Lang, C. H.; Hargrove, D. M.; Spitzer, J. J.

In: Journal of Applied Physiology, Vol. 67, No. 5, 01.12.1989, p. 1770-1775.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tissue glucose utilization during epinephrine-induced hyperglycemia

AU - Meszaros, K.

AU - Lang, C. H.

AU - Hargrove, D. M.

AU - Spitzer, J. J.

PY - 1989/12/1

Y1 - 1989/12/1

N2 - The aim of this study was to investigate glucose utilization by individual tissues during epinephrine infusion. First, the applicability of the 2-deoxyglucose (2-DG) tracer technique during in vivo hyperglycemia was investigated in model systems in vitro. Epitrochlearis muscle and spleen cells were incubated with 1.25-20 mM glucose. The discrimination against 2-[14C]DG in glucose metabolic pathways, expressed by the lumped constant, remained unchanged over this wide range of glucose concentrations. It was concluded that in vivo hyperglycemia does not preclude the application of the 2-DG method. In a series of in vivo experiments, chronically catheterized conscious rats fasted for 24 h and were infused with epinephrine (0.2 μg·kg-1·min-1), which produced a two-fold increase in plasma glucose concentration. 2-[14C]DG was injected 30 min after starting the epinephrine infusion and glucose utilization rates of individual tissues were calculated based on the concentration of phosphorylated 2-DG in samples excised at 70 min. The epinephrine infusion increased glucose utilization rates by 40-160% in hindlimb muscles, skin, ileum, liver, spleen, lung, epidydimal fat, and kidney, although no change was found in the brain. Mass action of the increased plasma glucose is likely to play an important role in the enhanced rate of glucose utilization.

AB - The aim of this study was to investigate glucose utilization by individual tissues during epinephrine infusion. First, the applicability of the 2-deoxyglucose (2-DG) tracer technique during in vivo hyperglycemia was investigated in model systems in vitro. Epitrochlearis muscle and spleen cells were incubated with 1.25-20 mM glucose. The discrimination against 2-[14C]DG in glucose metabolic pathways, expressed by the lumped constant, remained unchanged over this wide range of glucose concentrations. It was concluded that in vivo hyperglycemia does not preclude the application of the 2-DG method. In a series of in vivo experiments, chronically catheterized conscious rats fasted for 24 h and were infused with epinephrine (0.2 μg·kg-1·min-1), which produced a two-fold increase in plasma glucose concentration. 2-[14C]DG was injected 30 min after starting the epinephrine infusion and glucose utilization rates of individual tissues were calculated based on the concentration of phosphorylated 2-DG in samples excised at 70 min. The epinephrine infusion increased glucose utilization rates by 40-160% in hindlimb muscles, skin, ileum, liver, spleen, lung, epidydimal fat, and kidney, although no change was found in the brain. Mass action of the increased plasma glucose is likely to play an important role in the enhanced rate of glucose utilization.

UR - http://www.scopus.com/inward/record.url?scp=0024818270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024818270&partnerID=8YFLogxK

M3 - Article

C2 - 2600011

AN - SCOPUS:0024818270

VL - 67

SP - 1770

EP - 1775

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 5

ER -