Tissue-spanning redox gradient-dependent assembly of native human papillomavirus type 16 virions

Michael J. Conway, Samina Alam, Eric J. Ryndock, Linda Cruz, Neil D. Christensen, Richard B.S. Roden, Craig Meyers

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Papillomavirus capsids are composed of 72 pentamers reinforced through inter- and intrapentameric disulfide bonds. Recent research suggests that virus-like particles and pseudovirions (PsV) can undergo a redox-dependent conformational change involving disulfide interactions. We present here evidence that native virions exploit a tissue-spanning redox gradient that facilitates assembly events in the context of the complete papillomavirus life cycle. DNA encapsidation and infectivity titers are redox dependent in that they can be temporally modulated via treatment of organotypic cultures with oxidized glutathione. These data provide evidence that papillomavirus assembly and maturation is redox-dependent, utilizing multiple steps within both suprabasal and cornified layers.

Original languageEnglish (US)
Pages (from-to)10515-10526
Number of pages12
JournalJournal of virology
Volume83
Issue number20
DOIs
StatePublished - Oct 1 2009

Fingerprint

Human papillomavirus 16
Papillomaviridae
virion
Population Groups
Virion
Oxidation-Reduction
Disulfides
virus-like particles
capsid
disulfide bonds
sulfides
Glutathione Disulfide
glutathione
Capsid
pathogenicity
Life Cycle Stages
DNA
tissues
Research
Therapeutics

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Conway, Michael J. ; Alam, Samina ; Ryndock, Eric J. ; Cruz, Linda ; Christensen, Neil D. ; Roden, Richard B.S. ; Meyers, Craig. / Tissue-spanning redox gradient-dependent assembly of native human papillomavirus type 16 virions. In: Journal of virology. 2009 ; Vol. 83, No. 20. pp. 10515-10526.
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Tissue-spanning redox gradient-dependent assembly of native human papillomavirus type 16 virions. / Conway, Michael J.; Alam, Samina; Ryndock, Eric J.; Cruz, Linda; Christensen, Neil D.; Roden, Richard B.S.; Meyers, Craig.

In: Journal of virology, Vol. 83, No. 20, 01.10.2009, p. 10515-10526.

Research output: Contribution to journalArticle

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