Tissue-specific binding of a nuclear factor to the insulin gene promoter

Clare Sample, Donald F. Steiner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Using a DNA-binding gel mobility shift assay, a protein-DNA complex was detected using the rat II insulin promoter and a nuclear extract from rat insulinoma cells. This complex was detected using extracts from other insulin-producing cell lines but was not found using nuclear extracts from non-insulin-producing pancreatic cell lines or other cell types. Binding of the tissue-specific protein to the labelled promoter fragment was effectively competed by both rat and human insulin genes but not by the SV40 TATA box. This protein-DNA interaction may be involved in tissue-specific regulation of insulin gene expression.

Original languageEnglish (US)
Pages (from-to)332-336
Number of pages5
JournalFEBS Letters
Volume222
Issue number2
DOIs
StatePublished - Oct 5 1987

Fingerprint

Genes
Insulin
Tissue
Rats
DNA
Cells
Cell Line
TATA Box
Insulinoma
Proteins
Gene Expression Regulation
Electrophoretic Mobility Shift Assay
Gene expression
Assays
Gels

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Sample, Clare ; Steiner, Donald F. / Tissue-specific binding of a nuclear factor to the insulin gene promoter. In: FEBS Letters. 1987 ; Vol. 222, No. 2. pp. 332-336.
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Tissue-specific binding of a nuclear factor to the insulin gene promoter. / Sample, Clare; Steiner, Donald F.

In: FEBS Letters, Vol. 222, No. 2, 05.10.1987, p. 332-336.

Research output: Contribution to journalArticle

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