Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells

Jason D. Heaney, Ashley N. Rettew, Sarah K. Bronson

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The hypoxanthine phosphoribosyltransferase (Hprt) locus has been shown to have minimal influence on transgene expression when used as a surrogate site in the mouse genome. We have developed a method to transfer bacterial artificial chromosomes (BACs) as a single copy into the partially deleted Hprt locus of embryonic stem cells. BACs were modified by Cre/loxP recombination to contain the sequences necessary for homologous recombination into and complementation of the partially deleted Hprt locus. Modified BACs were shown to undergo homologous recombination into the genome intact, to be stably transmitted through the germ line of transgenic mice, and to be expressed in the proper tissue-specific manner. This technology will facilitate many studies in which correct interpretation of data depends on developmentally appropriate transgene expression in the absence of rearrangements or deletions of endogenous DNA.

Original languageEnglish (US)
Pages (from-to)1072-1082
Number of pages11
JournalGenomics
Volume83
Issue number6
DOIs
StatePublished - Jun 2004

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells'. Together they form a unique fingerprint.

Cite this