Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e

Curtis John Omiecinski

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The tissue-specific expression of cytochrome P-450b and P-450e mRNAs was examined with synthetic 18-mer oligomer probes in the liver, lung, kidney, and testis of control and inducer pretreated adult rats. RNAs homologous to the P-450e probe were detected in trace amounts in control and 3-methylcholanthrene (MC) induced livers and at high levels in livers from phenobarbital (PB) induced animals. P-450e mRNA levels were below detection limits in the other tissues examined, regardless of pretreatment. In contrast, mRNAs homologous to the P-450b oligomer were detected at low levels in control and inducer pretreated lung and testis, and at high levels in PB induced liver. No P-450b mRNAs were detected in these assays in RNA isolates from the kidney or from control or MC pretreated liver. Solution hybridization data indicated that the rat lung contained 9-12%, and the testis, 6-9%, respectively, of the levels of P-450b mRNA measured in the PB induced liver. Results from oligo(dT)-cellulose and poly(U)-affinity experiments indicated that the hepatic mRNAs for P-450b and P-450e were present predominantly in the bound, polyadenylated fraction, whereas the homologous lung and testes P-450b mRNAs predominated in the flow-thru fractions.

Original languageEnglish (US)
Pages (from-to)1525-1539
Number of pages15
JournalNucleic acids research
Volume14
Issue number3
DOIs
StatePublished - Feb 11 1986

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Cytochromes
Messenger RNA
Liver
Testis
Phenobarbital
Lung
Methylcholanthrene
RNA
Kidney
Poly U
Limit of Detection

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Omiecinski, Curtis John. / Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e. In: Nucleic acids research. 1986 ; Vol. 14, No. 3. pp. 1525-1539.
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Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e. / Omiecinski, Curtis John.

In: Nucleic acids research, Vol. 14, No. 3, 11.02.1986, p. 1525-1539.

Research output: Contribution to journalArticle

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