Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated

Suzy M. Cocciolone, Surinder Chopra, Sherry A. Flint-Garcia, Michael D. McMullen, Thomas Peterson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The maize p1 gene encodes a Myb-homologous regulator of red pigment biosynthesis. To investigate the tissue-specific regulation of the p1 gene, maize plants were transformed with constructs combining promoter and cDNA sequences of two alleles which differ in pigmentation patterns: P1-wr (white pericarp/red cob) and P1-rr (red pericarp/red cob). Surprisingly, all promoter/cDNA combinations produced transgenic plants with red pericarp and red cob (RR pattern), indicating that the P1-wr promoter and encoded protein can function in pericarp. Some of the RR patterned transgenic plants produced progeny plants with white pericarp and red cob (WR pattern), and this switch in tissue-specificity correlated with increased transgene methylation. A similar inverse correlation between pericarp pigmentation and DNA methylation was observed for certain natural p1 alleles, which have a gene structure characteristic of standard P1-wr alleles, but which confer red pericarp pigmentation and are consistently less methylated than standard P1-wr alleles. Although we cannot rule out the possible existence of tissue-specific regulatory elements within the p1 non-coding sequences or flanking regions, the data from transgenic and natural alleles suggest that the tissue-specific pigmentation pattern characteristic of the P1-wr phenotype is epigenetically controlled.

Original languageEnglish (US)
Pages (from-to)467-478
Number of pages12
JournalPlant Journal
Volume27
Issue number5
DOIs
StatePublished - Oct 20 2001

Fingerprint

pericarp
Zea mays
Pigmentation
Transcription Factors
transcription factors
Alleles
corn
pigmentation
alleles
Genetically Modified Plants
promoter regions
Complementary DNA
Plant Genes
Organ Specificity
transgenic plants
DNA Methylation
Transgenes
Methylation
Genes
genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Cocciolone, Suzy M. ; Chopra, Surinder ; Flint-Garcia, Sherry A. ; McMullen, Michael D. ; Peterson, Thomas. / Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated. In: Plant Journal. 2001 ; Vol. 27, No. 5. pp. 467-478.
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Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated. / Cocciolone, Suzy M.; Chopra, Surinder; Flint-Garcia, Sherry A.; McMullen, Michael D.; Peterson, Thomas.

In: Plant Journal, Vol. 27, No. 5, 20.10.2001, p. 467-478.

Research output: Contribution to journalArticle

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