Transgenic mice carrying multiple copies of a recoverable λ phage shuttle vector carrying the supF mutation reporter gene (λsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 × 10-5), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue-specific differences in genetic instability.
|Original language||English (US)|
|Number of pages||6|
|Journal||Environmental and Molecular Mutagenesis|
|Publication status||Published - Dec 1 1996|
All Science Journal Classification (ASJC) codes
- Health, Toxicology and Mutagenesis