Tissue Specificity of Spontaneous Point Mutations in λsupF transgenic Mice

Eric G. Leach, Latha Narayanan, Pamela A. Havre, Edward J. Gunther, Toni M. Yeasky, Peter M. Glazer

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Transgenic mice carrying multiple copies of a recoverable λ phage shuttle vector carrying the supF mutation reporter gene (λsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 × 10-5), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue-specific differences in genetic instability.

Original languageEnglish (US)
Pages (from-to)459-464
Number of pages6
JournalEnvironmental and Molecular Mutagenesis
Volume28
Issue number4
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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