Tissue targeting and plasma clearance of cobra venom factor in mice

Qinlan Fu, Pondichery G. Satyaswaroop, Channe Gowda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation with a plasma half life of about 10 h, whereas CVF injected into the peritoneal cavity was slowly absorbed into the blood stream reaching a maximum level at ~ 6 h, and it was then cleared from the circulation with a plasma half life of about 18 h. The rate of plasma clearance of CVF was markedly decreased upon removal of the terminal α-galactosyl residues of the oligosaccharide chains; the plasma half lives for intravenously and intraperitoneally administered de-α-galactosylated CVF were ~ 5 and ~ 10 h, respectively. However, the clearance rate was not affected by complete deglycosylation using N-glycanase or by chemical modification of the terminal galactosyl residues. Together, these data demonstrate that the terminal α-galactosyl residues of CVF mask the Lewis X-dependent uptake of CVF by liver.

Original languageEnglish (US)
Pages (from-to)316-320
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number2
DOIs
StatePublished - Feb 13 1997

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Tissue
Plasmas
Liver
Half-Life
Blood
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
cobra venom factor
Peritoneal Cavity
Chemical modification
Masks
Intraperitoneal Injections
Oligosaccharides
Intravenous Injections

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The tissue targeting and rate of clearance of cobra venom factor (CVF) from the circulation was studied in mice by intravenous or intraperitoneal injection of radioiodinated CVF. In both modes of administrations, CVF was targeted mainly to liver. CVF injected directly into the blood was cleared from the circulation with a plasma half life of about 10 h, whereas CVF injected into the peritoneal cavity was slowly absorbed into the blood stream reaching a maximum level at ~ 6 h, and it was then cleared from the circulation with a plasma half life of about 18 h. The rate of plasma clearance of CVF was markedly decreased upon removal of the terminal α-galactosyl residues of the oligosaccharide chains; the plasma half lives for intravenously and intraperitoneally administered de-α-galactosylated CVF were ~ 5 and ~ 10 h, respectively. However, the clearance rate was not affected by complete deglycosylation using N-glycanase or by chemical modification of the terminal galactosyl residues. Together, these data demonstrate that the terminal α-galactosyl residues of CVF mask the Lewis X-dependent uptake of CVF by liver.",
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Tissue targeting and plasma clearance of cobra venom factor in mice. / Fu, Qinlan; Satyaswaroop, Pondichery G.; Gowda, Channe.

In: Biochemical and Biophysical Research Communications, Vol. 231, No. 2, 13.02.1997, p. 316-320.

Research output: Contribution to journalArticle

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