TLR5 activation induces secretory interleukin-1 receptor antagonist (sIL-1Ra) and reduces inflammasome-associated tissue damage

F. A. Carvalho, J. D. Aitken, A. T. Gewirtz, Matam Vijay Kumar

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Toll-like receptor-5 (TLR5)-mediated detection of flagellin induces nuclear factor (NF)-B-mediated transcription of host defense gene expression, whereas recognition of intracellular flagellin by interleukin (IL)-1-converting enzyme protease-activation factor (IPAF) results in maturation/secretion of the inflammasome cytokine IL-1β. The potent effects of IL-1β are counter-regulated by secretory IL-1 receptor antagonist (sIL-1Ra). We studied the roles of flagellin receptors in regulating the expression of IL-1β and sIL-1Ra and their subsequent roles in inflammation. Flagellin induced sIL-1Ra in intestinal epithelia and macrophages in a dose- and time-dependent manner, whereas IL-1β was only induced in macrophages. In vivo, flagellin-induced sIL-1Ra, but not IL-1β, was absolutely dependent upon TLR5 expressed on non-hemopioetic cells. Thus, loss of TLR5 increased the IL-1β/sIL-1Ra ratio on flagellin treatment, which correlated with increased inflammatory pathology in response to this product. Furthermore, the flagellin/TLR5 interaction was important for the induction of sIL-1Ra and limiting inflammatory pathology on Salmonella infection. Finally, reduced sIL-1Ra levels in TLR5KO mice correlated with spontaneous colitis. Taken together, we demonstrate that intestinal epithelia, despite not expressing IL-1β, secrete sIL-1Ra in a TLR5-dependent manner suggesting that loss of TLR5 may promote inflammation by increasing IL-1β activity. Thus, optimizing the balance between inflammasome cytokines and their endogenous inhibitors might prove a useful strategy to treat inflammatory disorders.

Original languageEnglish (US)
Pages (from-to)102-111
Number of pages10
JournalMucosal Immunology
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2011

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Toll-Like Receptor 5
Inflammasomes
Interleukin-1 Receptors
Flagellin
Interleukin-1
Intestinal Mucosa
Macrophages
Pathology
Cytokines
Inflammation
Caspase 1
Enzyme Activation
Salmonella Infections
Colitis
Peptide Hydrolases
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Carvalho, F. A. ; Aitken, J. D. ; Gewirtz, A. T. ; Kumar, Matam Vijay. / TLR5 activation induces secretory interleukin-1 receptor antagonist (sIL-1Ra) and reduces inflammasome-associated tissue damage. In: Mucosal Immunology. 2011 ; Vol. 4, No. 1. pp. 102-111.
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TLR5 activation induces secretory interleukin-1 receptor antagonist (sIL-1Ra) and reduces inflammasome-associated tissue damage. / Carvalho, F. A.; Aitken, J. D.; Gewirtz, A. T.; Kumar, Matam Vijay.

In: Mucosal Immunology, Vol. 4, No. 1, 01.01.2011, p. 102-111.

Research output: Contribution to journalArticle

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AU - Aitken, J. D.

AU - Gewirtz, A. T.

AU - Kumar, Matam Vijay

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