tmRNA Is Essential in Shigella flexneri

Nitya S. Ramadoss, Xin Zhou, Kenneth Charles Keiler

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria.

Original languageEnglish (US)
Article numbere57537
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 25 2013

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Shigella flexneri
translation (genetics)
Escherichia coli
Bacteria
Messenger RNA
Gene encoding
Terminator Codon
Ribosomes
Sequence Analysis
Genes
stop codon
bacteria
Genome
ribosomes
tmRNA
sequence analysis
viability
Proteins
genome
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ramadoss, Nitya S. ; Zhou, Xin ; Keiler, Kenneth Charles. / tmRNA Is Essential in Shigella flexneri. In: PLoS One. 2013 ; Vol. 8, No. 2.
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tmRNA Is Essential in Shigella flexneri. / Ramadoss, Nitya S.; Zhou, Xin; Keiler, Kenneth Charles.

In: PLoS One, Vol. 8, No. 2, e57537, 25.02.2013.

Research output: Contribution to journalArticle

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