TNFα Induces E-Selectin Expression and PECAM-1 (CD31) Redistribution in Extracutaneous Tissues

M. D. Ioffreda, S. M. Albelda, D. E. Elder, A. Radu, L. C. Leventhal, B. Zweiman, G. F. Murphy

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We evaluated the experimental utility of short-term organ culture to assess cytokine-dependent endothelial activation in extracutaneous tissues. Effects of tumor necrosis factor-alpha (TNFα) on E-selectin and platelet-endothelial cell adhesion molecule-1 (PECAM-1) were assessed immunocytochemically in tissue derived from human gastrointestinal tract, female genital tract, breast, lung, kidney, and skin. Normal vessels in control explants from these sites did not stain for E-selectin and expressed PECAM-1 uniformly along plasma membranes of lumenal endothelial cells. Cytokine-stimulated vessels in explants exposed to TNFa for 6 hours revealed marked induction of E-selectin and redistribution of PECAM-1 to interendothelial junctions. PECAM-1 redistribution was also confirmed in situ using Type I skin allergic reactions in which mast cell TNF is presumably released. As in cytokine-stimulated skin, E-selectin expression in extracutaneous tissues was restricted to postcapillary venules, although anastomotic junctions between capillaries and venules were more clearly defined at extracutaneous sites. These data establish short-term organ culture as a potentially useful means of assessing endothelial activation in extracutaneous tissues.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalEndothelium
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1993

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

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