TonB System, In Vivo Assays and Characterization

Research output: Chapter in Book/Report/Conference proceedingChapter

28 Citations (Scopus)

Abstract

The multiprotein TonB system of Escherichia coli involves proteins in both the cytoplasmic membrane and the outer membrane. By a still unclear mechanism, the proton-motive force of the cytoplasmic membrane is used to catalyze active transport through high-affinity transporters in the outer membrane. TonB, ExbB, and ExbD are required to transduce the cytoplasmic membrane energy to these transporters. For E. coli, transport ligands consist of iron-siderophore complexes, vitamin B12, group B colicins, and bacteriophages T1 and ø80. Our experimental philosophy is that data gathered in vivo, where all known and unknown components are present at balanced chromosomal levels in the whole cell, can be interpreted with less ambiguity than when a subset of components is overexpressed or analysed in vitro. This chapter describes in vivo assays for the TonB system and their application.

Original languageEnglish (US)
Title of host publicationTwo Component Signaling Systems, Part A
PublisherAcademic Press Inc.
Pages245-269
Number of pages25
DOIs
StatePublished - Jan 1 2007

Publication series

NameMethods in Enzymology
Volume422
ISSN (Print)0076-6879

Fingerprint

Assays
Cell Membrane
Membranes
Colicins
Siderophores
Proton-Motive Force
Active Biological Transport
Escherichia coli Proteins
Escherichia coli
Vitamin B 12
Bacteriophages
Iron
Ligands
Protons
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Postle, K. (2007). TonB System, In Vivo Assays and Characterization. In Two Component Signaling Systems, Part A (pp. 245-269). (Methods in Enzymology; Vol. 422). Academic Press Inc.. https://doi.org/10.1016/S0076-6879(06)22012-3
Postle, Kathleen. / TonB System, In Vivo Assays and Characterization. Two Component Signaling Systems, Part A. Academic Press Inc., 2007. pp. 245-269 (Methods in Enzymology).
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Postle, K 2007, TonB System, In Vivo Assays and Characterization. in Two Component Signaling Systems, Part A. Methods in Enzymology, vol. 422, Academic Press Inc., pp. 245-269. https://doi.org/10.1016/S0076-6879(06)22012-3

TonB System, In Vivo Assays and Characterization. / Postle, Kathleen.

Two Component Signaling Systems, Part A. Academic Press Inc., 2007. p. 245-269 (Methods in Enzymology; Vol. 422).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - The multiprotein TonB system of Escherichia coli involves proteins in both the cytoplasmic membrane and the outer membrane. By a still unclear mechanism, the proton-motive force of the cytoplasmic membrane is used to catalyze active transport through high-affinity transporters in the outer membrane. TonB, ExbB, and ExbD are required to transduce the cytoplasmic membrane energy to these transporters. For E. coli, transport ligands consist of iron-siderophore complexes, vitamin B12, group B colicins, and bacteriophages T1 and ø80. Our experimental philosophy is that data gathered in vivo, where all known and unknown components are present at balanced chromosomal levels in the whole cell, can be interpreted with less ambiguity than when a subset of components is overexpressed or analysed in vitro. This chapter describes in vivo assays for the TonB system and their application.

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Postle K. TonB System, In Vivo Assays and Characterization. In Two Component Signaling Systems, Part A. Academic Press Inc. 2007. p. 245-269. (Methods in Enzymology). https://doi.org/10.1016/S0076-6879(06)22012-3