Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics

T. L. King, B. A. Lubinski, M. K. Burnham-Curtis, W. Stott, R. P. Morgan

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

We document isolation and characterization of 13 tri- and tetranucleotide microsatellite DNA markers in brook trout (Salvelinus fontinalis). These markers displayed moderate to high levels of allelic diversity (averaging 20.5 alleles/locus) and heterozygosity (averaging 53.5%) in a range-wide survey of more than 13,000 fish. A comparison of two geographically proximal populations located on opposite sides of the eastern continental divide in Maryland, USA, found no deviations from Hardy-Weinberg equilibrium and minimal linkage disequilibrium. Microsatellite markers developed for S. fontinalis yielded sufficient genetic diversity to: (1) produce unique multilocus genotypes; (2) elucidate phylogeographic structure; and (3) provide unique demographic perspectives of population sizes and historical demographics. This suite of markers also provided considerable cross-species amplification utility among related salmonids.

Original languageEnglish (US)
Pages (from-to)539-543
Number of pages5
JournalConservation Genetics Resources
Volume4
Issue number3
DOIs
StatePublished - Sep 2012

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Fingerprint

Dive into the research topics of 'Tools for the management and conservation of genetic diversity in brook trout (Salvelinus fontinalis): Tri- and tetranucleotide microsatellite markers for the assessment of genetic diversity, phylogeography, and historical demographics'. Together they form a unique fingerprint.

Cite this