Tooth transplantation in the mouse

The role of minor (non-H-2) histocompatibility loci in tooth germ transplantation

Jan Klein

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Tooth germs (first molars) of 4-5-day-old mice were transplanted to the s.c. connective tissue of adult recipients in the following strain combinations: B10 → B10.BY (H-l° difference), B10.BY→ B10 (H-lā difference), B10 → B10.LPa (H-3a difference), B10.LPa → B10 (H-3b difference), B10 → B10.129(21M) (H-4a difference), B10. 129 (21M) → (H-4b difference), and A.BY → BIO (multiple non-H-2 difference). The recipients were killed at different intervals from 5 to 280 days after transplantation and the grafts were examined histologically after standard hematoxylin and eosin staining and marking with Procion dyes. In all the single non-H-2 differences, the allografts behaved like syngeneic grafts and there was no evidence of immune allograft reaction. After a transient period of adjustment, during which a nonspecific inflammatory response took place, the tooth germs continued to develop and mature. The deviations from normal development could be attributed to the trauma caused by the surgical procedure and to the heterotopic environment of the grafts. Tooth germs transplanted across the multiple non-H-2 barrier (A.BY → BIO), on the other hand, showed evidence of relatively strong immune reaction. They were heavily infiltrated with mononuclear cells and their further development was either completely arrested or seriously distorted.

Original languageEnglish (US)
Pages (from-to)500-508
Number of pages9
JournalTransplantation
Volume12
Issue number6
DOIs
StatePublished - Jan 1 1971

Fingerprint

Tooth Germ
Histocompatibility
Tooth
Transplantation
Transplants
Allografts
Hematoxylin
Eosine Yellowish-(YS)
Connective Tissue
Coloring Agents
Staining and Labeling
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

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title = "Tooth transplantation in the mouse: The role of minor (non-H-2) histocompatibility loci in tooth germ transplantation",
abstract = "Tooth germs (first molars) of 4-5-day-old mice were transplanted to the s.c. connective tissue of adult recipients in the following strain combinations: B10 → B10.BY (H-l° difference), B10.BY→ B10 (H-lā difference), B10 → B10.LPa (H-3a difference), B10.LPa → B10 (H-3b difference), B10 → B10.129(21M) (H-4a difference), B10. 129 (21M) → (H-4b difference), and A.BY → BIO (multiple non-H-2 difference). The recipients were killed at different intervals from 5 to 280 days after transplantation and the grafts were examined histologically after standard hematoxylin and eosin staining and marking with Procion dyes. In all the single non-H-2 differences, the allografts behaved like syngeneic grafts and there was no evidence of immune allograft reaction. After a transient period of adjustment, during which a nonspecific inflammatory response took place, the tooth germs continued to develop and mature. The deviations from normal development could be attributed to the trauma caused by the surgical procedure and to the heterotopic environment of the grafts. Tooth germs transplanted across the multiple non-H-2 barrier (A.BY → BIO), on the other hand, showed evidence of relatively strong immune reaction. They were heavily infiltrated with mononuclear cells and their further development was either completely arrested or seriously distorted.",
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Tooth transplantation in the mouse : The role of minor (non-H-2) histocompatibility loci in tooth germ transplantation. / Klein, Jan.

In: Transplantation, Vol. 12, No. 6, 01.01.1971, p. 500-508.

Research output: Contribution to journalArticle

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