Abstract

Background There are over two million laparotomies performed in the United States each year with an incisional hernia rate between 2% and 11%. A total of 100,000 ventral hernia repairs are undertaken each year with recurrences as high as 50%. Materials and methods Full thickness midline fascia incisions from the xiphoid to the pubic symphysis were made in rats. The fascia and/or muscular layer was sutured closed and a gel with 300 μM of sodium orthovanadate or saline was placed over the suture line with the skin closed over it. On day 10, 1-cm strips from the superior, middle, and inferior regions of the abdominal wall were tested for breaking strength and processed for histology. Results The mean wound breaking strength of vanadate-treated wounds was 18.6 ± 2.7 N compared with 9.4 ± 3.6 N for controls (P < 0.0001). Similar quantities of granulation tissue were deposited in treated and control wounds. Fine green birefringence patterns, characteristic of immature connective tissue, were seen in control samples viewed with polarized light. In contrast, vanadate-treated wounds showed thick yellow-orange birefringence patterns characteristics of more mature connective tissue. Using α-smooth muscle actin immunostaining, myofibroblasts were prominent in control incisions, but few were identified in vanadate-treated incisions. Conclusions In rat laparotomy wounds, a single application of vanadate increases wound breaking strength, through enhanced connective tissue organization. These combined data suggest topical application of vanadate immediately after fascial closure will increase wound strength, possibly reducing hernia recurrences in the repaired abdominal wall.

Original languageEnglish (US)
Pages (from-to)102-107
Number of pages6
JournalJournal of Surgical Research
Volume207
DOIs
StatePublished - Jan 1 2017

Fingerprint

Vanadates
Laparotomy
Wounds and Injuries
Connective Tissue
Birefringence
Fascia
Abdominal Wall
Pubic Symphysis
Ventral Hernia
Recurrence
Myofibroblasts
Granulation Tissue
Herniorrhaphy
Hernia
Sutures
Smooth Muscle
Actins
Histology
Gels
Sodium

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

@article{735e6e32362548a99520982a45baceb3,
title = "Topical vanadate enhances the repair of median laparotomy incisions",
abstract = "Background There are over two million laparotomies performed in the United States each year with an incisional hernia rate between 2{\%} and 11{\%}. A total of 100,000 ventral hernia repairs are undertaken each year with recurrences as high as 50{\%}. Materials and methods Full thickness midline fascia incisions from the xiphoid to the pubic symphysis were made in rats. The fascia and/or muscular layer was sutured closed and a gel with 300 μM of sodium orthovanadate or saline was placed over the suture line with the skin closed over it. On day 10, 1-cm strips from the superior, middle, and inferior regions of the abdominal wall were tested for breaking strength and processed for histology. Results The mean wound breaking strength of vanadate-treated wounds was 18.6 ± 2.7 N compared with 9.4 ± 3.6 N for controls (P < 0.0001). Similar quantities of granulation tissue were deposited in treated and control wounds. Fine green birefringence patterns, characteristic of immature connective tissue, were seen in control samples viewed with polarized light. In contrast, vanadate-treated wounds showed thick yellow-orange birefringence patterns characteristics of more mature connective tissue. Using α-smooth muscle actin immunostaining, myofibroblasts were prominent in control incisions, but few were identified in vanadate-treated incisions. Conclusions In rat laparotomy wounds, a single application of vanadate increases wound breaking strength, through enhanced connective tissue organization. These combined data suggest topical application of vanadate immediately after fascial closure will increase wound strength, possibly reducing hernia recurrences in the repaired abdominal wall.",
author = "Hazard, {Sprague W.} and Zwemer, {Charles F.} and Mackay, {Donald R.} and Koduru, {Srinivas V.} and Ravnic, {Dino J.} and Ehrlich, {H. Paul}",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.jss.2016.08.078",
language = "English (US)",
volume = "207",
pages = "102--107",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",

}

Topical vanadate enhances the repair of median laparotomy incisions. / Hazard, Sprague W.; Zwemer, Charles F.; Mackay, Donald R.; Koduru, Srinivas V.; Ravnic, Dino J.; Ehrlich, H. Paul.

In: Journal of Surgical Research, Vol. 207, 01.01.2017, p. 102-107.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Topical vanadate enhances the repair of median laparotomy incisions

AU - Hazard, Sprague W.

AU - Zwemer, Charles F.

AU - Mackay, Donald R.

AU - Koduru, Srinivas V.

AU - Ravnic, Dino J.

AU - Ehrlich, H. Paul

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background There are over two million laparotomies performed in the United States each year with an incisional hernia rate between 2% and 11%. A total of 100,000 ventral hernia repairs are undertaken each year with recurrences as high as 50%. Materials and methods Full thickness midline fascia incisions from the xiphoid to the pubic symphysis were made in rats. The fascia and/or muscular layer was sutured closed and a gel with 300 μM of sodium orthovanadate or saline was placed over the suture line with the skin closed over it. On day 10, 1-cm strips from the superior, middle, and inferior regions of the abdominal wall were tested for breaking strength and processed for histology. Results The mean wound breaking strength of vanadate-treated wounds was 18.6 ± 2.7 N compared with 9.4 ± 3.6 N for controls (P < 0.0001). Similar quantities of granulation tissue were deposited in treated and control wounds. Fine green birefringence patterns, characteristic of immature connective tissue, were seen in control samples viewed with polarized light. In contrast, vanadate-treated wounds showed thick yellow-orange birefringence patterns characteristics of more mature connective tissue. Using α-smooth muscle actin immunostaining, myofibroblasts were prominent in control incisions, but few were identified in vanadate-treated incisions. Conclusions In rat laparotomy wounds, a single application of vanadate increases wound breaking strength, through enhanced connective tissue organization. These combined data suggest topical application of vanadate immediately after fascial closure will increase wound strength, possibly reducing hernia recurrences in the repaired abdominal wall.

AB - Background There are over two million laparotomies performed in the United States each year with an incisional hernia rate between 2% and 11%. A total of 100,000 ventral hernia repairs are undertaken each year with recurrences as high as 50%. Materials and methods Full thickness midline fascia incisions from the xiphoid to the pubic symphysis were made in rats. The fascia and/or muscular layer was sutured closed and a gel with 300 μM of sodium orthovanadate or saline was placed over the suture line with the skin closed over it. On day 10, 1-cm strips from the superior, middle, and inferior regions of the abdominal wall were tested for breaking strength and processed for histology. Results The mean wound breaking strength of vanadate-treated wounds was 18.6 ± 2.7 N compared with 9.4 ± 3.6 N for controls (P < 0.0001). Similar quantities of granulation tissue were deposited in treated and control wounds. Fine green birefringence patterns, characteristic of immature connective tissue, were seen in control samples viewed with polarized light. In contrast, vanadate-treated wounds showed thick yellow-orange birefringence patterns characteristics of more mature connective tissue. Using α-smooth muscle actin immunostaining, myofibroblasts were prominent in control incisions, but few were identified in vanadate-treated incisions. Conclusions In rat laparotomy wounds, a single application of vanadate increases wound breaking strength, through enhanced connective tissue organization. These combined data suggest topical application of vanadate immediately after fascial closure will increase wound strength, possibly reducing hernia recurrences in the repaired abdominal wall.

UR - http://www.scopus.com/inward/record.url?scp=84991264404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991264404&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2016.08.078

DO - 10.1016/j.jss.2016.08.078

M3 - Article

C2 - 27979464

AN - SCOPUS:84991264404

VL - 207

SP - 102

EP - 107

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

ER -