Topographic restriction of tag-1 expression in the developing retinotectal pathway and target dependent reexpression during axon regeneration

Dirk M. Lang, James Warren, Jr., Christiane Klisa, Claudia A.O. Stuermer

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

TAG-1, a glycosylphosphatidyl inositol (GPI)-anchored protein of the immunoglobulin (Ig) superfamily, exhibits an unusual spatiotemporal expression pattern in the fish visual pathway. Using in situ hybridization and new antibodies (Abs) against fish TAG-1 we show that TAG-1 mRNA and anti-TAG-1 staining is restricted to nasal retinal ganglion cells (RGCs) in 24- to 72-h-old zebrafish embryos and in the adult, continuously growing goldfish retina. Anti-TAG-1 Abs selectively label nasal RGC axons in the nerve, optic tract, and tectum. Axotomized RGCs reexpress TAG-1, which occurs as late as 12 days after optic nerve lesion, when regenerating RGC axons arrive in the tectum, suggesting TAG-1 reexpression is target contact-dependent. Accordingly, TAG-1 reexpression ceases upon interruption of the regenerating projection by a second lesion. The topographic restriction of TAG-1 expression and its target dependency during regeneration suggests that TAG-1 might pray a role in the retinotopic organization and restoration of the retinotectal pathway.

Original languageEnglish (US)
Pages (from-to)398-414
Number of pages17
JournalMolecular and Cellular Neuroscience
Volume17
Issue number2
DOIs
StatePublished - Jan 1 2001

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Retinal Ganglion Cells
Axons
Regeneration
Nose
Fishes
Goldfish
Visual Pathways
Superior Colliculi
Antibodies
Zebrafish
Inositol
Optic Nerve
In Situ Hybridization
Retina
Immunoglobulins
Embryonic Structures
Staining and Labeling
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

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Topographic restriction of tag-1 expression in the developing retinotectal pathway and target dependent reexpression during axon regeneration. / Lang, Dirk M.; Warren, Jr., James; Klisa, Christiane; Stuermer, Claudia A.O.

In: Molecular and Cellular Neuroscience, Vol. 17, No. 2, 01.01.2001, p. 398-414.

Research output: Contribution to journalArticle

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