Toward a mechanistic and physiological understanding of a ferredoxin:disulfide reductase from the domains Archaea and Bacteria

Divya Prakash, Karim A. Walters, X. Ryan J. Martinie, Addison C. McCarver, Adepu K. Kumar, Daniel J. Lessner, Carsten Krebs, John H. Golbeck, James Gregory Ferry

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Disulfide reductases reduce other proteins and are critically important for cellular redox signaling and homeostasis. Methanosarcina acetivoransis a methane-producing microbe fromthe domain Archaea that produces a ferredoxin:disulfide reductase (FDR) for which the crystal structure has been reported, yet its biochemical mechanism and physiological substrates are unknown. FDR and the extensively characterized plant-type ferredoxin:thioredoxin reductase (FTR) belong to a distinct class of disulfide reductases that contain a unique active-site [4Fe-4S]+ cluster. The results reported here support a mechanism for FDR similar to that reported for FTR with notable exceptions. Unlike FTR, FDR contains a rubredoxin [1Fe-0S] center postulated to mediate electron transfer from ferredoxin to the active-site [4Fe-4S]+ cluster. UV-visible, EPR, and Mo?ssbauer spectroscopic data indicated that two-electron reduction of the active-site disulfide in FDR involves a one-electron-reduced [4Fe-4S]+ intermediate previously hypothesized for FTR. Our results support a role for an active-site tyrosine in FDR that occupies the equivalent position of an essential histidine in the active site of FTR. Of note, one of seven Trxs encoded in the genome (Trx5) and methanoredoxin, a glutaredoxin-like enzyme from M. acetivorans, were reduced by FDR, advancing the physiological understanding of FDR?s role in the redox metabolism of methanoarchaea. Finally, bioinformatics analyses show that FDR homologs are widespread in diverse microbes from the domain Bacteria.

Original languageEnglish (US)
Pages (from-to)9198-9209
Number of pages12
JournalJournal of Biological Chemistry
Volume293
Issue number24
DOIs
StatePublished - Jan 1 2018

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Ferredoxins
Archaea
Disulfides
Bacteria
Oxidoreductases
Catalytic Domain
Electrons
Oxidation-Reduction
Rubredoxins
Methanosarcina
Glutaredoxins
Methane
Bioinformatics
Computational Biology
Histidine
Metabolism
Paramagnetic resonance
Tyrosine
Homeostasis
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Prakash, Divya ; Walters, Karim A. ; Martinie, X. Ryan J. ; McCarver, Addison C. ; Kumar, Adepu K. ; Lessner, Daniel J. ; Krebs, Carsten ; Golbeck, John H. ; Ferry, James Gregory. / Toward a mechanistic and physiological understanding of a ferredoxin:disulfide reductase from the domains Archaea and Bacteria. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 24. pp. 9198-9209.
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abstract = "Disulfide reductases reduce other proteins and are critically important for cellular redox signaling and homeostasis. Methanosarcina acetivoransis a methane-producing microbe fromthe domain Archaea that produces a ferredoxin:disulfide reductase (FDR) for which the crystal structure has been reported, yet its biochemical mechanism and physiological substrates are unknown. FDR and the extensively characterized plant-type ferredoxin:thioredoxin reductase (FTR) belong to a distinct class of disulfide reductases that contain a unique active-site [4Fe-4S]+ cluster. The results reported here support a mechanism for FDR similar to that reported for FTR with notable exceptions. Unlike FTR, FDR contains a rubredoxin [1Fe-0S] center postulated to mediate electron transfer from ferredoxin to the active-site [4Fe-4S]+ cluster. UV-visible, EPR, and Mo?ssbauer spectroscopic data indicated that two-electron reduction of the active-site disulfide in FDR involves a one-electron-reduced [4Fe-4S]+ intermediate previously hypothesized for FTR. Our results support a role for an active-site tyrosine in FDR that occupies the equivalent position of an essential histidine in the active site of FTR. Of note, one of seven Trxs encoded in the genome (Trx5) and methanoredoxin, a glutaredoxin-like enzyme from M. acetivorans, were reduced by FDR, advancing the physiological understanding of FDR?s role in the redox metabolism of methanoarchaea. Finally, bioinformatics analyses show that FDR homologs are widespread in diverse microbes from the domain Bacteria.",
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Toward a mechanistic and physiological understanding of a ferredoxin:disulfide reductase from the domains Archaea and Bacteria. / Prakash, Divya; Walters, Karim A.; Martinie, X. Ryan J.; McCarver, Addison C.; Kumar, Adepu K.; Lessner, Daniel J.; Krebs, Carsten; Golbeck, John H.; Ferry, James Gregory.

In: Journal of Biological Chemistry, Vol. 293, No. 24, 01.01.2018, p. 9198-9209.

Research output: Contribution to journalArticle

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T1 - Toward a mechanistic and physiological understanding of a ferredoxin:disulfide reductase from the domains Archaea and Bacteria

AU - Prakash, Divya

AU - Walters, Karim A.

AU - Martinie, X. Ryan J.

AU - McCarver, Addison C.

AU - Kumar, Adepu K.

AU - Lessner, Daniel J.

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AU - Ferry, James Gregory

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N2 - Disulfide reductases reduce other proteins and are critically important for cellular redox signaling and homeostasis. Methanosarcina acetivoransis a methane-producing microbe fromthe domain Archaea that produces a ferredoxin:disulfide reductase (FDR) for which the crystal structure has been reported, yet its biochemical mechanism and physiological substrates are unknown. FDR and the extensively characterized plant-type ferredoxin:thioredoxin reductase (FTR) belong to a distinct class of disulfide reductases that contain a unique active-site [4Fe-4S]+ cluster. The results reported here support a mechanism for FDR similar to that reported for FTR with notable exceptions. Unlike FTR, FDR contains a rubredoxin [1Fe-0S] center postulated to mediate electron transfer from ferredoxin to the active-site [4Fe-4S]+ cluster. UV-visible, EPR, and Mo?ssbauer spectroscopic data indicated that two-electron reduction of the active-site disulfide in FDR involves a one-electron-reduced [4Fe-4S]+ intermediate previously hypothesized for FTR. Our results support a role for an active-site tyrosine in FDR that occupies the equivalent position of an essential histidine in the active site of FTR. Of note, one of seven Trxs encoded in the genome (Trx5) and methanoredoxin, a glutaredoxin-like enzyme from M. acetivorans, were reduced by FDR, advancing the physiological understanding of FDR?s role in the redox metabolism of methanoarchaea. Finally, bioinformatics analyses show that FDR homologs are widespread in diverse microbes from the domain Bacteria.

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