Toward imaging the dynamic interaction between actin and myosin using a wet-cell C-mode near-field optical microscope

J. P. Stitt, R. L. Tutwiler, Venkatraman Gopalan, L. Larrson

Research output: Contribution to journalConference article

Abstract

This paper describes the implementation of a wet-cell collection-mode near-field optical microscope that has been constructed with the long term goal of imaging the myosin conformational change as it interacts with actin. The bioassay used to prepare the samples involved lysing muscle fibers to exact and myosin molecules which were fixed to a mica slide. Next, actin filaments were exacted and deposited in the region of the myosin. The wet-cell allows the addition of water containing ATP and the necessary ions to activate the myosin biomolecules. The near-field optical microscope was shown to able to resolve features of the size of the actin and myosin biomolecules.

Original languageEnglish (US)
Pages (from-to)5250-5253
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 VII
StatePublished - Dec 1 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

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Biomolecules
Myosins
Actins
Microscopes
Imaging techniques
Bioassay
Adenosinetriphosphate
Mica
Muscle
Molecules
Fibers
Ions
Water
Actin Cytoskeleton
Biological Assay
Adenosine Triphosphate
Muscles

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

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AU - Gopalan, Venkatraman

AU - Larrson, L.

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