Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography

Michael C. Breadmore, Sushil Shrinivasan, James Michael Karlinsey, Jerome P. Ferrance, Pamela M. Norris, James P. Landers

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The potential for using polyelectrolyte multilayers (PEMs) to provide chromatographic functionality on continuous silica networks created from sol-gel chemistry has been evaluated by capillary electrochromatography (CEC). Construction of the PEM was achieved by flushing the column with polyelectrolytes of alternative charge, with variation of the properties of the exposed polyelectrolyte providing a unique means to vary the chromatographic surface. Variation of the exposed polyelectrolyte from poly(diallyldimethylammonium chloride) (PDDAC) to dextran sulfate (DS) allowed the direction of the electroosmotic flow (EOF) to be changed and also provided a means to vary the chromatographic capacity. Variation of negative polymer from DS to poly(styrene sulfonate) (PSS) significantly altered the EOF and the migration of peptides, with both the reversed-phase and ion-exchange capacities increasing. An alternative method for changing the column capacity was to change the thickness of the PEM, which was evaluated by anion-exchange CEC. A 70-80% increase in retention was observed for all anions without any increase in EOF suggesting significant penetration of the analytes through the PEM and interaction with buried charges within the PEM.

Original languageEnglish (US)
Pages (from-to)1261-1270
Number of pages10
JournalELECTROPHORESIS
Volume24
Issue number7-8
StatePublished - Apr 1 2003

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Capillary Electrochromatography
Polymethyl Methacrylate
Polyelectrolytes
Sol-gels
Multilayers
Gels
Electroosmosis
Dextran Sulfate
Anions
Ion exchange
Styrene
Ion Exchange
Silicon Dioxide
Polymers
Peptides

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Breadmore, Michael C. ; Shrinivasan, Sushil ; Karlinsey, James Michael ; Ferrance, Jerome P. ; Norris, Pamela M. ; Landers, James P. / Towards a microchip-based chromatographic platform. Part 2 : Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography. In: ELECTROPHORESIS. 2003 ; Vol. 24, No. 7-8. pp. 1261-1270.
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Towards a microchip-based chromatographic platform. Part 2 : Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography. / Breadmore, Michael C.; Shrinivasan, Sushil; Karlinsey, James Michael; Ferrance, Jerome P.; Norris, Pamela M.; Landers, James P.

In: ELECTROPHORESIS, Vol. 24, No. 7-8, 01.04.2003, p. 1261-1270.

Research output: Contribution to journalArticle

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