Towards Sharka containment, the 'Sharco' project: Genetic approach

E. M. Vera-Ruiz, C. Romero, G. Llácer, M. L. Badenes, T. Zhebentyayeva, A. Abbott

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sharka disease, caused by Plum Pox Virus (PPV), produces important economic losses in Apricot (P. armeniaca L.). PPV natural resistance has been only found in some North American apricot cultivars and efforts are being made to introduce this character on Mediterranean germplasm. Recent studies on segregation of different intraspecific apricot crosses suggest that PPV resistance is controlled by at least one major dominant locus on the upper part of linkage group 1 (LG1). Using microsatellites already mapped in different Prunus spp. we have built a high density genetic map for an interval of 13 cM comprising this region in apricot. In addition, overgo probes designed from SSR clone sequences have been used to screen an apricot Bacterial Artificial Chromosome (BAC) library. Positive clones identified are being used to construct a BAC contig spanning the apricot genomic region containing the PPV resistance locus using the peach physical map developed by Zhebentyayeva et al. (2008) as reference.

Original languageEnglish (US)
Title of host publicationActa Horticulturae
Pages471-476
Number of pages6
StatePublished - May 31 2010

Publication series

NameActa Horticulturae
Volume862
ISSN (Print)0567-7572

Fingerprint

apricots
Plum pox virus
bacterial artificial chromosomes
Prunus
clones
loci
physical chromosome mapping
linkage groups
peaches
germplasm
microsatellite repeats
genomics
economics
cultivars

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Vera-Ruiz, E. M., Romero, C., Llácer, G., Badenes, M. L., Zhebentyayeva, T., & Abbott, A. (2010). Towards Sharka containment, the 'Sharco' project: Genetic approach. In Acta Horticulturae (pp. 471-476). (Acta Horticulturae; Vol. 862).
Vera-Ruiz, E. M. ; Romero, C. ; Llácer, G. ; Badenes, M. L. ; Zhebentyayeva, T. ; Abbott, A. / Towards Sharka containment, the 'Sharco' project : Genetic approach. Acta Horticulturae. 2010. pp. 471-476 (Acta Horticulturae).
@inproceedings{9229b47e8a69447cb382a5da0a7cb406,
title = "Towards Sharka containment, the 'Sharco' project: Genetic approach",
abstract = "Sharka disease, caused by Plum Pox Virus (PPV), produces important economic losses in Apricot (P. armeniaca L.). PPV natural resistance has been only found in some North American apricot cultivars and efforts are being made to introduce this character on Mediterranean germplasm. Recent studies on segregation of different intraspecific apricot crosses suggest that PPV resistance is controlled by at least one major dominant locus on the upper part of linkage group 1 (LG1). Using microsatellites already mapped in different Prunus spp. we have built a high density genetic map for an interval of 13 cM comprising this region in apricot. In addition, overgo probes designed from SSR clone sequences have been used to screen an apricot Bacterial Artificial Chromosome (BAC) library. Positive clones identified are being used to construct a BAC contig spanning the apricot genomic region containing the PPV resistance locus using the peach physical map developed by Zhebentyayeva et al. (2008) as reference.",
author = "Vera-Ruiz, {E. M.} and C. Romero and G. Ll{\'a}cer and Badenes, {M. L.} and T. Zhebentyayeva and A. Abbott",
year = "2010",
month = "5",
day = "31",
language = "English (US)",
isbn = "9789066053564",
series = "Acta Horticulturae",
pages = "471--476",
booktitle = "Acta Horticulturae",

}

Vera-Ruiz, EM, Romero, C, Llácer, G, Badenes, ML, Zhebentyayeva, T & Abbott, A 2010, Towards Sharka containment, the 'Sharco' project: Genetic approach. in Acta Horticulturae. Acta Horticulturae, vol. 862, pp. 471-476.

Towards Sharka containment, the 'Sharco' project : Genetic approach. / Vera-Ruiz, E. M.; Romero, C.; Llácer, G.; Badenes, M. L.; Zhebentyayeva, T.; Abbott, A.

Acta Horticulturae. 2010. p. 471-476 (Acta Horticulturae; Vol. 862).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Towards Sharka containment, the 'Sharco' project

T2 - Genetic approach

AU - Vera-Ruiz, E. M.

AU - Romero, C.

AU - Llácer, G.

AU - Badenes, M. L.

AU - Zhebentyayeva, T.

AU - Abbott, A.

PY - 2010/5/31

Y1 - 2010/5/31

N2 - Sharka disease, caused by Plum Pox Virus (PPV), produces important economic losses in Apricot (P. armeniaca L.). PPV natural resistance has been only found in some North American apricot cultivars and efforts are being made to introduce this character on Mediterranean germplasm. Recent studies on segregation of different intraspecific apricot crosses suggest that PPV resistance is controlled by at least one major dominant locus on the upper part of linkage group 1 (LG1). Using microsatellites already mapped in different Prunus spp. we have built a high density genetic map for an interval of 13 cM comprising this region in apricot. In addition, overgo probes designed from SSR clone sequences have been used to screen an apricot Bacterial Artificial Chromosome (BAC) library. Positive clones identified are being used to construct a BAC contig spanning the apricot genomic region containing the PPV resistance locus using the peach physical map developed by Zhebentyayeva et al. (2008) as reference.

AB - Sharka disease, caused by Plum Pox Virus (PPV), produces important economic losses in Apricot (P. armeniaca L.). PPV natural resistance has been only found in some North American apricot cultivars and efforts are being made to introduce this character on Mediterranean germplasm. Recent studies on segregation of different intraspecific apricot crosses suggest that PPV resistance is controlled by at least one major dominant locus on the upper part of linkage group 1 (LG1). Using microsatellites already mapped in different Prunus spp. we have built a high density genetic map for an interval of 13 cM comprising this region in apricot. In addition, overgo probes designed from SSR clone sequences have been used to screen an apricot Bacterial Artificial Chromosome (BAC) library. Positive clones identified are being used to construct a BAC contig spanning the apricot genomic region containing the PPV resistance locus using the peach physical map developed by Zhebentyayeva et al. (2008) as reference.

UR - http://www.scopus.com/inward/record.url?scp=77957298141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957298141&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:77957298141

SN - 9789066053564

T3 - Acta Horticulturae

SP - 471

EP - 476

BT - Acta Horticulturae

ER -

Vera-Ruiz EM, Romero C, Llácer G, Badenes ML, Zhebentyayeva T, Abbott A. Towards Sharka containment, the 'Sharco' project: Genetic approach. In Acta Horticulturae. 2010. p. 471-476. (Acta Horticulturae).