Abstract
A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays with HD-1 spore, CryIA(a), CryIA(b), CryIA(c), CryIB, CryIC, CryID, CryIE, or CryIIA. The data revealed high levels of field- evolved resistance to HD-1 spore and all CryIA pentoxins and no resistance to CryIB, CryIC, or CryID. CryIE and CryIIA were essentially nut toxic. When HD- 1 spore was combined 1:1 with protoxin and fed to susceptible larvae, spore synergized the activity of CryIA and CryIC 5- to 8-fold and 1.7-fold, respectively, and did not synergize the mortality of CryIIA. When fed to Florida larvae, spore failed to synergize the activity of all three CryIA protoxins, synergized the activity of CryIC 5.3-fold, and did not synergize the mortality for CryIIA. Binding studies with CryIA(b), CryIB, and CryIC were performed to determine possible mechanisms of resistance. The two techniques used were (i) binding of biotinylated toxin to tissue sections larval midguts and (ii) binding of biotinylated toxin to brush border membrane vesicles prepared from whole larvae. Both showed dramatically reduced binding of CryIA(b) in resistant larvae compared with that in susceptible larvae but no differences in binding of CryIB or CryIC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 564-569 |
| Number of pages | 6 |
| Journal | Applied and Environmental Microbiology |
| Volume | 62 |
| Issue number | 2 |
| State | Published - Feb 1 1996 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology
Cite this
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Toxicity of Bacillus thuringiensis spore and crystal protein to resistant diamondback moth (Plutella xylostella). / Tang, Juliet D.; Shelton, Anthony M.; Van Rie, Jeroen; De Roeck, Sandra; Moar, William J.; Roush, Richard T.; Peferoen, Marnix.
In: Applied and Environmental Microbiology, Vol. 62, No. 2, 01.02.1996, p. 564-569.Research output: Contribution to journal › Article
TY - JOUR
T1 - Toxicity of Bacillus thuringiensis spore and crystal protein to resistant diamondback moth (Plutella xylostella)
AU - Tang, Juliet D.
AU - Shelton, Anthony M.
AU - Van Rie, Jeroen
AU - De Roeck, Sandra
AU - Moar, William J.
AU - Roush, Richard T.
AU - Peferoen, Marnix
PY - 1996/2/1
Y1 - 1996/2/1
N2 - A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays with HD-1 spore, CryIA(a), CryIA(b), CryIA(c), CryIB, CryIC, CryID, CryIE, or CryIIA. The data revealed high levels of field- evolved resistance to HD-1 spore and all CryIA pentoxins and no resistance to CryIB, CryIC, or CryID. CryIE and CryIIA were essentially nut toxic. When HD- 1 spore was combined 1:1 with protoxin and fed to susceptible larvae, spore synergized the activity of CryIA and CryIC 5- to 8-fold and 1.7-fold, respectively, and did not synergize the mortality of CryIIA. When fed to Florida larvae, spore failed to synergize the activity of all three CryIA protoxins, synergized the activity of CryIC 5.3-fold, and did not synergize the mortality for CryIIA. Binding studies with CryIA(b), CryIB, and CryIC were performed to determine possible mechanisms of resistance. The two techniques used were (i) binding of biotinylated toxin to tissue sections larval midguts and (ii) binding of biotinylated toxin to brush border membrane vesicles prepared from whole larvae. Both showed dramatically reduced binding of CryIA(b) in resistant larvae compared with that in susceptible larvae but no differences in binding of CryIB or CryIC.
AB - A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays with HD-1 spore, CryIA(a), CryIA(b), CryIA(c), CryIB, CryIC, CryID, CryIE, or CryIIA. The data revealed high levels of field- evolved resistance to HD-1 spore and all CryIA pentoxins and no resistance to CryIB, CryIC, or CryID. CryIE and CryIIA were essentially nut toxic. When HD- 1 spore was combined 1:1 with protoxin and fed to susceptible larvae, spore synergized the activity of CryIA and CryIC 5- to 8-fold and 1.7-fold, respectively, and did not synergize the mortality of CryIIA. When fed to Florida larvae, spore failed to synergize the activity of all three CryIA protoxins, synergized the activity of CryIC 5.3-fold, and did not synergize the mortality for CryIIA. Binding studies with CryIA(b), CryIB, and CryIC were performed to determine possible mechanisms of resistance. The two techniques used were (i) binding of biotinylated toxin to tissue sections larval midguts and (ii) binding of biotinylated toxin to brush border membrane vesicles prepared from whole larvae. Both showed dramatically reduced binding of CryIA(b) in resistant larvae compared with that in susceptible larvae but no differences in binding of CryIB or CryIC.
UR - http://www.scopus.com/inward/record.url?scp=0030027837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030027837&partnerID=8YFLogxK
M3 - Article
VL - 62
SP - 564
EP - 569
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 2
ER -