Toxin YafQ increases persister cell formation by reducing indole signalling

Ying Hu, Brian W. Kwan, Devon O. Osbourne, Michael J. Benedik, Thomas Keith Wood

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Summary: Persister cells survive antibiotic and other environmental stresses by slowing metabolism. Since toxins of toxin/antitoxin (TA) systems have been postulated to be responsible for persister cell formation, we investigated the influence of toxin YafQ of the YafQ/DinJ Escherichia coli TA system on persister cell formation. Under stress, YafQ alters metabolism by cleaving transcripts with in-frame 5′-AAA-G/A-3′ sites. Production of YafQ increased persister cell formation with multiple antibiotics, and by investigating changes in protein expression, we found that YafQ reduced tryptophanase levels (TnaA mRNA has 16 putative YafQ cleavage sites). Consistently, TnaA mRNA levels were also reduced by YafQ. Tryptophanase is activated in the stationary phase by the stationary-phase sigma factor RpoS, which was also reduced dramatically upon production of YafQ. Tryptophanase converts tryptophan into indole, and as expected, indole levels were reduced by the production of YafQ. Corroborating the effect of YafQ on persistence, addition of indole reduced persistence. Furthermore, persistence increased upon deleting tnaA, and persistence decreased upon adding tryptophan to the medium to increase indole levels. Also, YafQ production had a much smaller effect on persistence in a strain unable to produce indole. Therefore, YafQ increases persistence by reducing indole, and TA systems are related to cell signalling.

Original languageEnglish (US)
Pages (from-to)1275-1285
Number of pages11
JournalEnvironmental microbiology
Volume17
Issue number4
DOIs
StatePublished - Apr 1 2015

Fingerprint

indoles
toxin
Tryptophanase
toxins
persistence
tryptophan 2,3-dioxygenase
antitoxins
Antitoxins
cells
Tryptophan
antibiotics
tryptophan
metabolism
Anti-Bacterial Agents
sigma factors
Sigma Factor
Messenger RNA
environmental stress
cleavage
indole

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hu, Ying ; Kwan, Brian W. ; Osbourne, Devon O. ; Benedik, Michael J. ; Wood, Thomas Keith. / Toxin YafQ increases persister cell formation by reducing indole signalling. In: Environmental microbiology. 2015 ; Vol. 17, No. 4. pp. 1275-1285.
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Toxin YafQ increases persister cell formation by reducing indole signalling. / Hu, Ying; Kwan, Brian W.; Osbourne, Devon O.; Benedik, Michael J.; Wood, Thomas Keith.

In: Environmental microbiology, Vol. 17, No. 4, 01.04.2015, p. 1275-1285.

Research output: Contribution to journalArticle

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