Trace metal and vitamin requirements of Methanococcoides methylutens grown with trimethylamine

Kevin R. Sowers, James Gregory Ferry

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Trace organic nutrient and metal requirements for the growth of Methanococcoides methylutens strain TMA-10 were determined in defined medium that contained trimethylamine as the substrate. Biotin was the only organic supplement required in place of yeast extract, Trypticase or a mixture of 8 B-vitamins. Fe an Ni were required for growth and low concentrations of Fe2+ (<5 μM) and Ni2+ (<0.25 μM) provided limited growth. In the absence of added Co the growth rate was reduced by 94% and growth was limited at concentrations below 0.1 μM. Stimulation of growth by Se, Mo, B, Al, Zn, Mn or Cu could not be demonstrated.

Original languageEnglish (US)
Pages (from-to)148-151
Number of pages4
JournalArchives of Microbiology
Volume142
Issue number2
DOIs
StatePublished - Jul 1 1985

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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