TY - JOUR
T1 - Tracheal aspirate transcriptomic and miRNA signatures of extreme premature birth with bronchopulmonary dysplasia
AU - Oji-Mmuo, Christiana N.
AU - Siddaiah, Roopa
AU - Montes, Deborah T.
AU - Pham, Melody A.
AU - Spear, Debra
AU - Donnelly, Ann
AU - Fuentes, Nathalie
AU - Imamura-Kawasawa, Yuka
AU - Howrylak, Judie A.
AU - Thomas, Neal J.
AU - Silveyra, Patricia
N1 - Funding Information:
Funding This work was supported by grants from Children’s Miracle Network (PS, CNO), Center for Research for Women and Newborn Health (PS, NF, RS), and Penn State College of Medicine faculty endowment funds (CNO, PS, RS).
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3
Y1 - 2021/3
N2 - Objective: Extreme preterm infants are a growing population in neonatal intensive care units who carry a high mortality and morbidity. Multiple factors play a role in preterm birth, resulting in major impact on organogenesis leading to complications including bronchopulmonary dysplasia (BPD). The goal of this study was to identify biomarker signatures associated with prematurity and BPD. Study design: We analyzed miRNA and mRNA profiles in tracheal aspirates (TAs) from 55 infants receiving invasive mechanical ventilation. Twenty-eight infants were extremely preterm and diagnosed with BPD, and 27 were term babies receiving invasive mechanical ventilation for elective procedures. Result: We found 22 miRNAs and 33 genes differentially expressed (FDR < 0.05) in TAs of extreme preterm infants with BPD vs. term babies without BPD. Pathway analysis showed associations with inflammatory response, cellular growth/proliferation, and tissue development. Conclusions: Specific mRNA-miRNA signatures in TAs may serve as biomarkers for BPD pathogenesis, a consequence of extreme prematurity.
AB - Objective: Extreme preterm infants are a growing population in neonatal intensive care units who carry a high mortality and morbidity. Multiple factors play a role in preterm birth, resulting in major impact on organogenesis leading to complications including bronchopulmonary dysplasia (BPD). The goal of this study was to identify biomarker signatures associated with prematurity and BPD. Study design: We analyzed miRNA and mRNA profiles in tracheal aspirates (TAs) from 55 infants receiving invasive mechanical ventilation. Twenty-eight infants were extremely preterm and diagnosed with BPD, and 27 were term babies receiving invasive mechanical ventilation for elective procedures. Result: We found 22 miRNAs and 33 genes differentially expressed (FDR < 0.05) in TAs of extreme preterm infants with BPD vs. term babies without BPD. Pathway analysis showed associations with inflammatory response, cellular growth/proliferation, and tissue development. Conclusions: Specific mRNA-miRNA signatures in TAs may serve as biomarkers for BPD pathogenesis, a consequence of extreme prematurity.
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U2 - 10.1038/s41372-020-00868-9
DO - 10.1038/s41372-020-00868-9
M3 - Article
C2 - 33177681
AN - SCOPUS:85095810960
VL - 41
SP - 551
EP - 561
JO - Journal of Perinatology
JF - Journal of Perinatology
SN - 0743-8346
IS - 3
ER -