Tracing origins of the salmonella bareilly strain causing a food-borne outbreak in the United States

Maria Hoffmann, Yan Luo, Steven R. Monday, Narjol Gonzalez-Escalona, Andrea R. Ottesen, Tim Muruvanda, Charles Wang, George Kastanis, Christine Keys, Daniel Janies, Izzet F. Senturk, Umit V. Catalyurek, Hua Wang, Thomas S. Hammack, William J. Wolfgang, Dianna Schoonmaker-Bopp, Alvina Chu, Robert Myers, Julie Haendiges, Peter S. EvansJianghong Meng, Errol A. Strain, Marc W. Allard, Eric W. Brown

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Background. Using a novel combination of whole-genome sequencing (WGS) analysis and geographic metadata, we traced the origins of Salmonella Bareilly isolates collected in 2012 during a widespread food-borne outbreak in the United States associated with scraped tuna imported from India. Methods. Using next-generation sequencing, we sequenced the complete genome of 100 Salmonella Bareilly isolates obtained from patients who consumed contaminated product, from natural sources, and from unrelated historically and geographically disparate foods. Pathogen genomes were linked to geography by projecting the phylogeny on a virtual globe and produced a transmission network. Results. Phylogenetic analysis of WGS data revealed a common origin for outbreak strains, indicating that patients in Maryland and New York were infected from sources originating at a facility in India. Conclusions. These data represent the first report fully integrating WGS analysis with geographic mapping and a novel use of transmission networks. Results showed that WGS vastly improves our ability to delimit the scope and source of bacterial food-borne contamination events. Furthermore, these findings reinforce the extraordinary utility that WGS brings to global outbreak investigation as a greatly enhanced approach to protecting the human food supply chain as well as public health in general.

Original languageEnglish (US)
Pages (from-to)502-508
Number of pages7
JournalJournal of Infectious Diseases
Volume213
Issue number4
DOIs
StatePublished - Feb 15 2016

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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