Trans-species polymorphism of class II Mhc loci in danio fishes

Robert Graser, Colm O'hUigin, Vladimir Vincek, Axel Meyer, Jan Klein

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

A characteristic feature of the major histocompatibility complex (Mhc) polymorphism in mammals is the existence of allelic lineages shared by related species. This trans-species polymorphism has thus far been documented only in primates, rodents, and artiodactyls. In this communication we provide evidence that it also exists in cyprinid (bony) fishes at the class II A and B loci coding for the α and β polypeptide chains of the class II α:β heterodimers. The study has focused on three species of the family Cyprinidae, subfamily Rasborinae: the zebrafish (Danio rerio), the giant danio (D. malabaricus), and the pearl danio (D, albolineatus). The polymerase chain reaction was used to amplify and then sequence intron 1 and exon 2 of the class II B loci and exon 2 of the class II A loci in these species. Phylogenetic analysis of the sequences revealed the existence of allelic lineages whose divergence predates the divergence of the three species at both the A and B loci. The lineages at the B locus in particular are separated by large genetic distances. The polymorphism is concentrated in the peptide-binding region sites and is apparently maintained by balancing selection. Sharing of this unique Mhc feature by both bony fishes and mammals suggests that the main function of the Mhc (presentation of peptides to T lymphocytes) has not changed during the last 400 million years of its evolution.

Original languageEnglish (US)
Pages (from-to)36-48
Number of pages13
JournalImmunogenetics
Volume44
Issue number1
DOIs
StatePublished - Jan 1 1996

Fingerprint

Major Histocompatibility Complex
Fishes
Zebrafish
Mammals
Exons
Peptide T
Cyprinidae
Peptides
Prednisolone
Introns
Primates
Sequence Analysis
Rodentia
Binding Sites
T-Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Graser, R., O'hUigin, C., Vincek, V., Meyer, A., & Klein, J. (1996). Trans-species polymorphism of class II Mhc loci in danio fishes. Immunogenetics, 44(1), 36-48. https://doi.org/10.1007/BF02602655
Graser, Robert ; O'hUigin, Colm ; Vincek, Vladimir ; Meyer, Axel ; Klein, Jan. / Trans-species polymorphism of class II Mhc loci in danio fishes. In: Immunogenetics. 1996 ; Vol. 44, No. 1. pp. 36-48.
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Graser, R, O'hUigin, C, Vincek, V, Meyer, A & Klein, J 1996, 'Trans-species polymorphism of class II Mhc loci in danio fishes', Immunogenetics, vol. 44, no. 1, pp. 36-48. https://doi.org/10.1007/BF02602655

Trans-species polymorphism of class II Mhc loci in danio fishes. / Graser, Robert; O'hUigin, Colm; Vincek, Vladimir; Meyer, Axel; Klein, Jan.

In: Immunogenetics, Vol. 44, No. 1, 01.01.1996, p. 36-48.

Research output: Contribution to journalArticle

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Graser R, O'hUigin C, Vincek V, Meyer A, Klein J. Trans-species polymorphism of class II Mhc loci in danio fishes. Immunogenetics. 1996 Jan 1;44(1):36-48. https://doi.org/10.1007/BF02602655