Transcription factor TFIID recognizes DNA sequences downstream of the TATA element in the Hsp70 heat shock gene

P. A. Emanuel, D. S. Gilmour

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA- containing and TATA-less promoters.

Original languageEnglish (US)
Pages (from-to)8449-8453
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
DOIs
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • General

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