Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression

Zhiqiang Zhang, Alicia Klatt, Andrew J. Henderson, David S. Gilmour

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Many elongation factors in eukaryotes promote gene expression by increasing the processivity of RNA polymerase II (Pol II). However, the stability of RNA Pol II elongation complexes suggests that such complexes are not inherently prone to prematurely terminating transcription, particularly at physiological nucleotide concentrations. We show that the termination factor, Pcf11, causes premature termination on an HIV provirus. The transcription that occurs when Pcf11 is depleted from cells or an extract is no longer sensitive to 6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB), a compound that causes premature termination. Hence, Pcf11 can act as a negative elongation factor to repress RNA Pol II gene expression in eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)1609-1614
Number of pages6
JournalGenes and Development
Volume21
Issue number13
DOIs
StatePublished - Jul 1 2007

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All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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