Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM

Joakim Mark Andersen, Rodolphe Barrangou, Maher Abou Hachem, Sampo J. Lahtinen, Yong Jun Goh, Birte Svensson, Todd R. Klaenhammer

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The human gastrointestinal tract can be positively modulated by dietary supplementation of probiotic bacteria in combination with prebiotic carbohydrates. Here differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β- linked galactosides and glucosides. These oligosaccharides induced genes encoding phosphoenolpyruvate-dependent sugar phosphotransferase systems (PTS), galactoside pentose hexuronide (GPH) permease, and ATP-binding cassette (ABC) transporters. PTS systems were upregulated primarily by di- and tri-saccharides such as cellobiose, isomaltose, isomaltulose, panose and gentiobiose, while ABC transporters were upregulated by raffinose, Polydextrose, and stachyose. A single GPH transporter was induced by lactitol and galactooligosaccharides (GOS). The various transporters were associated with a number of glycoside hydrolases from families 1, 2, 4, 13, 32, 36, 42, and 65, involved in the catabolism of various α- and β-linked glucosides and galactosides. Further subfamily specialization was also observed for different PTS-associated GH1 6-phospho-β-glucosidases implicated in the catabolism of gentiobiose and cellobiose. These findings highlight the broad oligosaccharide metabolic repertoire of L. acidophilus NCFM and establish a platform for selection and screening of both probiotic bacteria and prebiotic compounds that may positively influence the gastrointestinal microbiota.

Original languageEnglish (US)
Article numbere44409
JournalPloS one
Volume7
Issue number9
DOIs
StatePublished - Sep 19 2012

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Lactobacillus acidophilus
Galactosides
Prebiotics
galactosides
prebiotics
gentiobiose
Pentoses
uptake mechanisms
Cellobiose
phosphotransferases (kinases)
cellobiose
polydextrose
ABC transporters
metabolism
Phosphotransferases
ATP-Binding Cassette Transporters
pentoses
Glucosides
Probiotics
carbohydrates

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Andersen, J. M., Barrangou, R., Hachem, M. A., Lahtinen, S. J., Goh, Y. J., Svensson, B., & Klaenhammer, T. R. (2012). Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM. PloS one, 7(9), [e44409]. https://doi.org/10.1371/journal.pone.0044409
Andersen, Joakim Mark ; Barrangou, Rodolphe ; Hachem, Maher Abou ; Lahtinen, Sampo J. ; Goh, Yong Jun ; Svensson, Birte ; Klaenhammer, Todd R. / Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM. In: PloS one. 2012 ; Vol. 7, No. 9.
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Andersen, JM, Barrangou, R, Hachem, MA, Lahtinen, SJ, Goh, YJ, Svensson, B & Klaenhammer, TR 2012, 'Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM', PloS one, vol. 7, no. 9, e44409. https://doi.org/10.1371/journal.pone.0044409

Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM. / Andersen, Joakim Mark; Barrangou, Rodolphe; Hachem, Maher Abou; Lahtinen, Sampo J.; Goh, Yong Jun; Svensson, Birte; Klaenhammer, Todd R.

In: PloS one, Vol. 7, No. 9, e44409, 19.09.2012.

Research output: Contribution to journalArticle

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Andersen JM, Barrangou R, Hachem MA, Lahtinen SJ, Goh YJ, Svensson B et al. Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM. PloS one. 2012 Sep 19;7(9). e44409. https://doi.org/10.1371/journal.pone.0044409