Transcriptional factor mutations reveal regulatory complexities of heat shock and newly identified stress genes in Saccharomyces cerevisiae

Janet M. Treger, Anthony P. Schmitt, John R. Simon, Kevin McEntee

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

A computer-aided pattern search of the entire yeast genome was designed and used to identify 186 putative stress response element-regulated genes in Saccharomyces cerevisiae. Transcript levels of eight of these candidate genes were examined, and three (37%) were shown to be heat shock- and DNA damage- inducible and to require the Msn2p and Msn4p transcriptional activators for stress regulation. Significantly, several heat shock protein (HSP) genes were identified in this computer search. Using a series of single and multiple regulatory mutants, we demonstrate unexpected regulatory complexities among the HSP genes from S. cerevisiae following heat shock.

Original languageEnglish (US)
Pages (from-to)26875-26879
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number41
DOIs
StatePublished - Oct 9 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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