Transcriptional response of human microglial cells to interferon-γ

R. B. Rock, S. Hu, A. Deshpande, S. Munir, B. J. May, C. A. Baker, P. K. Peterson, V. Kapur

Research output: Contribution to journalArticle

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Abstract

Microglia, the resident macrophages in the central nervous system (CNS), play a pivotal role in innate and adaptive immune responses in the brain. The immune functions of microglia are regulated by cytokines, including interferon (IFN)-γ, which is a major mediator of macrophage activation. We describe the transcriptional profile of human fetal microglial cells at 1, 6, and 24h after IFN-γ treatment. The results show a change in the expression of 405 genes including transcriptionally induced chemokines, IFN-γ signaling factors, and major histocompatibility complex genes. Our results demonstrate that activation of microglia by IFN-γ induces proinflammatory T-lymphocyte-related chemokine genes as well as genes involved in antigen presentation. As a result, signals for T-cell infiltration and antigen presentation are produced to allow for microglia-T-cell interactions that likely contribute to defense against invading pathogens. In sum, our results provide a foundation for the molecular mechanisms of the microglial response to IFN-γ - a key to understanding cell-mediated immunity of the CNS.

Original languageEnglish (US)
Pages (from-to)712-719
Number of pages8
JournalGenes and Immunity
Volume6
Issue number8
DOIs
StatePublished - Dec 1 2005

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Interferons
Microglia
Antigen Presentation
T-Lymphocytes
Chemokines
Central Nervous System
Genes
Macrophage Activation
Viral Tumor Antigens
Adaptive Immunity
Major Histocompatibility Complex
Innate Immunity
Cellular Immunity
Cell Communication
Macrophages
Cytokines
Gene Expression
Brain

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics
  • Genetics(clinical)

Cite this

Rock, R. B., Hu, S., Deshpande, A., Munir, S., May, B. J., Baker, C. A., ... Kapur, V. (2005). Transcriptional response of human microglial cells to interferon-γ. Genes and Immunity, 6(8), 712-719. https://doi.org/10.1038/sj.gene.6364246
Rock, R. B. ; Hu, S. ; Deshpande, A. ; Munir, S. ; May, B. J. ; Baker, C. A. ; Peterson, P. K. ; Kapur, V. / Transcriptional response of human microglial cells to interferon-γ. In: Genes and Immunity. 2005 ; Vol. 6, No. 8. pp. 712-719.
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Rock, RB, Hu, S, Deshpande, A, Munir, S, May, BJ, Baker, CA, Peterson, PK & Kapur, V 2005, 'Transcriptional response of human microglial cells to interferon-γ', Genes and Immunity, vol. 6, no. 8, pp. 712-719. https://doi.org/10.1038/sj.gene.6364246

Transcriptional response of human microglial cells to interferon-γ. / Rock, R. B.; Hu, S.; Deshpande, A.; Munir, S.; May, B. J.; Baker, C. A.; Peterson, P. K.; Kapur, V.

In: Genes and Immunity, Vol. 6, No. 8, 01.12.2005, p. 712-719.

Research output: Contribution to journalArticle

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Rock RB, Hu S, Deshpande A, Munir S, May BJ, Baker CA et al. Transcriptional response of human microglial cells to interferon-γ. Genes and Immunity. 2005 Dec 1;6(8):712-719. https://doi.org/10.1038/sj.gene.6364246