Transcriptional response of Malus to Erwinia amylovora infection

J. L. Norelli, C. L. Bassett, D. A. Lalli, M. E. Wisniewski, Robert E. Farrell, Jr., A. M. Baldo, H. S. Aldwinckle

Research output: Contribution to journalConference article

Abstract

Suppression subtractive cDNA hybridization (SSH) was used to identify genes that are differentially up- and down-regulated in apples (Malus × domestica) in response to challenge with Erwinia amylovora (Ea). cDNA libraries were constructed from E. amylovora- and mock-challenged 'Gale Gala' apple leaf tissue at various time intervals after challenge treatment, ranging from 1 to 72 hours post inoculation (hpi) and utilized in SSH. A total of 432 non-redundant Malus ESTs isolated by SSH in response to E. amylovora challenge were characterized by bioinformatic analysis. Many ESTs identified following Ea-challenge of apple were similar to genes previously reported to respond to bacterial challenge in Arabidopsis thaliana. The results indicate that there was a substantial early (1 and 2 hpi) transcriptional response in apple to fire blight disease involving both the down- and up-regulation of host genes. Additionally, genes identified responding to fire blight challenge early (1 and 2 hpi) differed from those identified later (24, 48, 72 hpi) in the infection process. For example, within the defense/stress functional category, ESTs representing several different PR proteins were first detected 48 hpi (up-regulated), whereas earlier defense/stress ESTs were primarily associated with oxidative and osmotic stress.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalActa Horticulturae
Volume793
DOIs
StatePublished - Jan 1 2008
Event11th International Workshop on Fire Blight - Portland, OR, United States
Duration: Aug 12 2007Aug 17 2007

Fingerprint

Erwinia amylovora
Malus
apples
infection
hybridization
genes
Malus domestica
osmotic stress
bioinformatics
cDNA libraries
oxidative stress
Arabidopsis thaliana
leaves
proteins

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Norelli, J. L., Bassett, C. L., Lalli, D. A., Wisniewski, M. E., Farrell, Jr., R. E., Baldo, A. M., & Aldwinckle, H. S. (2008). Transcriptional response of Malus to Erwinia amylovora infection. Acta Horticulturae, 793, 189-194. https://doi.org/10.17660/ActaHortic.2008.793.26
Norelli, J. L. ; Bassett, C. L. ; Lalli, D. A. ; Wisniewski, M. E. ; Farrell, Jr., Robert E. ; Baldo, A. M. ; Aldwinckle, H. S. / Transcriptional response of Malus to Erwinia amylovora infection. In: Acta Horticulturae. 2008 ; Vol. 793. pp. 189-194.
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abstract = "Suppression subtractive cDNA hybridization (SSH) was used to identify genes that are differentially up- and down-regulated in apples (Malus × domestica) in response to challenge with Erwinia amylovora (Ea). cDNA libraries were constructed from E. amylovora- and mock-challenged 'Gale Gala' apple leaf tissue at various time intervals after challenge treatment, ranging from 1 to 72 hours post inoculation (hpi) and utilized in SSH. A total of 432 non-redundant Malus ESTs isolated by SSH in response to E. amylovora challenge were characterized by bioinformatic analysis. Many ESTs identified following Ea-challenge of apple were similar to genes previously reported to respond to bacterial challenge in Arabidopsis thaliana. The results indicate that there was a substantial early (1 and 2 hpi) transcriptional response in apple to fire blight disease involving both the down- and up-regulation of host genes. Additionally, genes identified responding to fire blight challenge early (1 and 2 hpi) differed from those identified later (24, 48, 72 hpi) in the infection process. For example, within the defense/stress functional category, ESTs representing several different PR proteins were first detected 48 hpi (up-regulated), whereas earlier defense/stress ESTs were primarily associated with oxidative and osmotic stress.",
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Norelli, JL, Bassett, CL, Lalli, DA, Wisniewski, ME, Farrell, Jr., RE, Baldo, AM & Aldwinckle, HS 2008, 'Transcriptional response of Malus to Erwinia amylovora infection', Acta Horticulturae, vol. 793, pp. 189-194. https://doi.org/10.17660/ActaHortic.2008.793.26

Transcriptional response of Malus to Erwinia amylovora infection. / Norelli, J. L.; Bassett, C. L.; Lalli, D. A.; Wisniewski, M. E.; Farrell, Jr., Robert E.; Baldo, A. M.; Aldwinckle, H. S.

In: Acta Horticulturae, Vol. 793, 01.01.2008, p. 189-194.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Transcriptional response of Malus to Erwinia amylovora infection

AU - Norelli, J. L.

AU - Bassett, C. L.

AU - Lalli, D. A.

AU - Wisniewski, M. E.

AU - Farrell, Jr., Robert E.

AU - Baldo, A. M.

AU - Aldwinckle, H. S.

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Y1 - 2008/1/1

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AB - Suppression subtractive cDNA hybridization (SSH) was used to identify genes that are differentially up- and down-regulated in apples (Malus × domestica) in response to challenge with Erwinia amylovora (Ea). cDNA libraries were constructed from E. amylovora- and mock-challenged 'Gale Gala' apple leaf tissue at various time intervals after challenge treatment, ranging from 1 to 72 hours post inoculation (hpi) and utilized in SSH. A total of 432 non-redundant Malus ESTs isolated by SSH in response to E. amylovora challenge were characterized by bioinformatic analysis. Many ESTs identified following Ea-challenge of apple were similar to genes previously reported to respond to bacterial challenge in Arabidopsis thaliana. The results indicate that there was a substantial early (1 and 2 hpi) transcriptional response in apple to fire blight disease involving both the down- and up-regulation of host genes. Additionally, genes identified responding to fire blight challenge early (1 and 2 hpi) differed from those identified later (24, 48, 72 hpi) in the infection process. For example, within the defense/stress functional category, ESTs representing several different PR proteins were first detected 48 hpi (up-regulated), whereas earlier defense/stress ESTs were primarily associated with oxidative and osmotic stress.

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