Transcriptional response of Pasteurella multocida to defined iron sources

Michael L. Paustian, Barbara J. May, Dongwei Cao, Daniel Boley, Vivek Kapur

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. This resulted in a set of data containing over 338,000 gene expression observations. On average, 12% of P. multocida genes were differentially expressed under any single condition. A majority of these genes encoded P. multocida proteins that were involved in either transport and binding or were annotated as hypothetical proteins. Several trends are evident when the data from different iron sources are compared. In general, only two genes (ptsN and sapD) were expressed at elevated levels under all of the conditions tested. The results also show that genes with increased expression in the presence of hemoglobin did not respond to transferrin or ferritin as an iron source. Correspondingly, genes with increased expression in the transferrin and ferritin experiments were expressed at reduced levels when hemoglobin was supplied as the sole iron source. Finally, the data show that genes that were most responsive to the presence of ferric citrate did not follow a trend similar to that of the other iron sources, suggesting that different pathways respond to inorganic or organic sources of iron in P. multocida. Taken together, our results demonstrate that unique subsets of P. multocida genes are expressed in response to different iron sources and that many of these genes have yet to be functionally characterized.

Original languageEnglish (US)
Pages (from-to)6714-6720
Number of pages7
JournalJournal of bacteriology
Volume184
Issue number23
DOIs
StatePublished - Dec 1 2002

Fingerprint

Pasteurella multocida
Iron
Genes
Transferrin
Ferritins
Hemoglobins
Gene Expression
Oligonucleotide Array Sequence Analysis
Proteins
Genome

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Paustian, Michael L. ; May, Barbara J. ; Cao, Dongwei ; Boley, Daniel ; Kapur, Vivek. / Transcriptional response of Pasteurella multocida to defined iron sources. In: Journal of bacteriology. 2002 ; Vol. 184, No. 23. pp. 6714-6720.
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Transcriptional response of Pasteurella multocida to defined iron sources. / Paustian, Michael L.; May, Barbara J.; Cao, Dongwei; Boley, Daniel; Kapur, Vivek.

In: Journal of bacteriology, Vol. 184, No. 23, 01.12.2002, p. 6714-6720.

Research output: Contribution to journalArticle

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