Transcriptional response of Pasteurella multocida to nutrient limitation

Michael L. Paustian, Barbara J. May, Vivek Kapur

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minimal media. Our analysis showed that the levels of expression of a total of 669 genes, representing approximately one-third of the genome, were detectably altered over the course of the experiment. A large number (n = 439) of genes, including those involved in energy metabolism, transport, protein synthesis, and binding, were expressed at higher levels in rich medium, suggesting that, upon exposure to a rich environment, P. multocida immediately begins to turn on many energy-intensive biosynthetic pathways or, conversely, turns these genes off when it is exposed to a nutrient-deficient environment. Genes with increased expression in minimal medium (n = 230) included those encoding amino acid biosynthesis and transport systems, outer membrane proteins, and heat shock proteins. Importantly, our analysis also identified a large number (n = 164) of genes with unknown functions whose expression was altered during nutrient limitation. Overall, the results of our study show that a wide repertoire of genes, many of which have yet to be functionally classified, undergo transcriptional regulation in P. multocida in response to growth in minimal medium and provide a strong foundation to investigate the transcriptional response of this multispecies pathogen to growth in a nutrient-limited environment.

Original languageEnglish (US)
Pages (from-to)3734-3739
Number of pages6
JournalJournal of bacteriology
Volume184
Issue number13
DOIs
StatePublished - Jun 24 2002

Fingerprint

Pasteurella multocida
Food
Genes
Growth
Genome
Amino Acid Transport Systems
Biosynthetic Pathways
Heat-Shock Proteins
Protein Binding
Energy Metabolism
Carrier Proteins
Membrane Proteins
Bacteria
Gene Expression

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Paustian, Michael L. ; May, Barbara J. ; Kapur, Vivek. / Transcriptional response of Pasteurella multocida to nutrient limitation. In: Journal of bacteriology. 2002 ; Vol. 184, No. 13. pp. 3734-3739.
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Transcriptional response of Pasteurella multocida to nutrient limitation. / Paustian, Michael L.; May, Barbara J.; Kapur, Vivek.

In: Journal of bacteriology, Vol. 184, No. 13, 24.06.2002, p. 3734-3739.

Research output: Contribution to journalArticle

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