Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites

Scott E. Lindner, Kristian E. Swearingen, Melanie J. Shears, Michael P. Walker, Erin N. Vrana, Kevin J. Hart, Allen M. Minns, Photini Sinnis, Robert L. Moritz, Stefan H.I. Kappe

Research output: Contribution to journalArticle

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Abstract

Plasmodium sporozoites are transmitted from infected mosquitoes to mammals, and must navigate the host skin and vasculature to infect the liver. This journey requires distinct proteomes. Here, we report the dynamic transcriptomes and proteomes of both oocyst sporozoites and salivary gland sporozoites in both rodent-infectious Plasmodium yoelii parasites and human-infectious Plasmodium falciparum parasites. The data robustly define mRNAs and proteins that are upregulated in oocyst sporozoites (UOS) or upregulated in infectious sporozoites (UIS) within the salivary glands, including many that are essential for sporozoite functions in the vector and host. Moreover, we find that malaria parasites use two overlapping, extensive, and independent programs of translational repression across sporozoite maturation to temporally regulate protein expression. Together with gene-specific validation experiments, these data indicate that two waves of translational repression are implemented and relieved at different times during sporozoite maturation, migration and infection, thus promoting their successful development and vector-to-host transition.

Original languageEnglish (US)
Article number4964
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

parasites
Sporozoites
proteome
Proteomics
Malaria
salivary glands
Parasites
Proteome
proteins
rodents
glands
mammals
Mammals
infectious diseases
liver
genes
Liver
Oocysts
Skin
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Lindner, Scott E. ; Swearingen, Kristian E. ; Shears, Melanie J. ; Walker, Michael P. ; Vrana, Erin N. ; Hart, Kevin J. ; Minns, Allen M. ; Sinnis, Photini ; Moritz, Robert L. ; Kappe, Stefan H.I. / Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites. In: Nature communications. 2019 ; Vol. 10, No. 1.
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Lindner, SE, Swearingen, KE, Shears, MJ, Walker, MP, Vrana, EN, Hart, KJ, Minns, AM, Sinnis, P, Moritz, RL & Kappe, SHI 2019, 'Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites', Nature communications, vol. 10, no. 1, 4964. https://doi.org/10.1038/s41467-019-12936-6

Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites. / Lindner, Scott E.; Swearingen, Kristian E.; Shears, Melanie J.; Walker, Michael P.; Vrana, Erin N.; Hart, Kevin J.; Minns, Allen M.; Sinnis, Photini; Moritz, Robert L.; Kappe, Stefan H.I.

In: Nature communications, Vol. 10, No. 1, 4964, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Swearingen, Kristian E.

AU - Shears, Melanie J.

AU - Walker, Michael P.

AU - Vrana, Erin N.

AU - Hart, Kevin J.

AU - Minns, Allen M.

AU - Sinnis, Photini

AU - Moritz, Robert L.

AU - Kappe, Stefan H.I.

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