Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)

Lua Lopez, Emily S. Bellis, Eric Wafula, Sarah J. Hearne, Loren Honaas, Paula E. Ralph, Michael P. Timko, Nnanna Unachukwu, Claude W. Depamphilis, Jesse R. Lasky

Research output: Contribution to journalArticle

Abstract

Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host-parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host-parasite interactions beyond the haustorial connection.

Original languageEnglish (US)
Pages (from-to)397-411
Number of pages15
JournalWeed Science
Volume67
Issue number4
DOIs
StatePublished - Jul 1 2019

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Striga hermonthica
parasitic plants
transcriptomics
parasites
Striga
host-parasite relationships
nutrient transport
genes
crop losses
grain sorghum
Sub-Saharan Africa
Sorghum bicolor
plant hormones

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Lopez, Lua ; Bellis, Emily S. ; Wafula, Eric ; Hearne, Sarah J. ; Honaas, Loren ; Ralph, Paula E. ; Timko, Michael P. ; Unachukwu, Nnanna ; Depamphilis, Claude W. ; Lasky, Jesse R. / Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica). In: Weed Science. 2019 ; Vol. 67, No. 4. pp. 397-411.
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Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica). / Lopez, Lua; Bellis, Emily S.; Wafula, Eric; Hearne, Sarah J.; Honaas, Loren; Ralph, Paula E.; Timko, Michael P.; Unachukwu, Nnanna; Depamphilis, Claude W.; Lasky, Jesse R.

In: Weed Science, Vol. 67, No. 4, 01.07.2019, p. 397-411.

Research output: Contribution to journalArticle

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